Preparation and immunoprotection of subgroup B avian leukosis virus inactivated vaccine

文献类型: 外文期刊

第一作者: Li, Xue

作者: Li, Xue;Dong, Xuan;Sun, Xiaolong;Li, Weihua;Zhao, Peng;Cui, Zhizhong;Li, Weihua;Wang, Xin

作者机构:

关键词: Avian leukosis virus subgroup B;Inactivated vaccine;Immune protection;Maternal antibodies;p27

期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )

ISSN: 0264-410X

年卷期: 2013 年 31 卷 46 期

页码:

收录情况: SCI

摘要: Objectives: To develop an inactivated vaccine against subgroup B avian leukosis virus (ALV-B) and determine if vaccination of chicken breeders could protect young chicks from ALV-B horizontal infection at early stage and accelerate eradication progress. Methods: Chicken embryo fibroblast (CEF) cells were inoculated with SDAU09C2 strain of ALV-B and ALV-CEF was inactivated for preparation of oil-adjuvant vaccine. Eggs were collected from un-vaccinated and 9 vaccinated great parent female chickens for incubation. 1-day-old chicks were bled for testing their maternal antibodies to ALV-A/B and then inoculated with ALV-B. Viremia and cloaca p27 detection dynamics were tested and compared between chick groups with or without maternal antibody to ALV. Results: In 3 weeks after 3 vaccination with the inactivated vaccine, all 9 vaccinated breeders developed high antibody titers against ALV-A/B with ELISA read values of 1.69-1.89 (the positive base line was 0.4) and kept at the high titers for at least another 4 weeks. Maternal antibody was detected in 70% (12/17) of chicks from breeders with high antibody titers to ALV-A/B. Only 4 of 12 chickens with maternal antibodies developed temporary viremia and no viremia was detected in the left 8 maternal antibody positive chickens during the whole 14 week after inoculation of ALV-B at 1 day of age. But the persistent viremia was detected in 2-8 weeks in all 9 maternal antibody negative chickens and the viremia persisted in the whole tested period of 14 weeks after inoculation of ALV-B. Conclusions: The inactivated ALV-B vaccine could induce high titer antibody reaction to ALV-B, it could provide maternal antibodies to 1-day-old chickens and protect chickens from early infection of ALV-B. (C) 2013 Elsevier Ltd. All rights reserved.

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