Identifying the candidate genes involved in the calyx abscission process of 'Kuerlexiangli' (Pyrus sinkiangensis Yu) by digital transcript abundance measurements

文献类型： 外文期刊

第一作者： Qi, Xiaoxiao

作者： Qi, Xiaoxiao;Wu, Jun;Wang, Lifen;Li, Leiting;Zhang, Shaoling;Cao, Yufen;Tian, Luming;Dong, Xingguang

作者机构：

关键词： Pear;Calyx abscission;Digital transcript abundance measurements;Differentially expressed genes

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2013 年 14 卷

页码：

收录情况： SCI

摘要： Background: 'Kuerlexiangli' (Pyrus sinkiangensis Yu), a native pear of Xinjiang, China, is an important agricultural fruit and primary export to the international market. However, fruit with persistent calyxes affect fruit shape and quality. Although several studies have looked into the physiological aspects of the calyx abscission process, the underlying molecular mechanisms remain unknown. In order to better understand the molecular basis of the process of calyx abscission, materials at three critical stages of regulation, with 6000 x Flusilazole plus 300 x PBO treatment (calyx abscising treatment) and 50 mg.L(-1)GA(3) treatment (calyx persisting treatment), were collected and cDNA fragments were sequenced using digital transcript abundance measurements to identify candidate genes. Results: Digital transcript abundance measurements was performed using high-throughput Illumina GAII sequencing on seven samples that were collected at three important stages of the calyx abscission process with chemical agent treatments promoting calyx abscission and persistence. Altogether more than 251,123,845 high quality reads were obtained with approximately 8.0 M raw data for each library. The values of 69.85%-71.90% of clean data in the digital transcript abundance measurements could be mapped to the pear genome database. There were 12,054 differentially expressed genes having Gene Ontology (GO) terms and associating with 251 Kyoto Encyclopedia of Genes and Genomes (KEGG) defined pathways. The differentially expressed genes correlated with calyx abscission were mainly involved in photosynthesis, plant hormone signal transduction, cell wall modification, transcriptional regulation, and carbohydrate metabolism. Furthermore, candidate calyx abscission-specific genes, e.g. Inflorescence deficient in abscission gene, were identified. Quantitative real-time PCR was used to confirm the digital transcript abundance measurements results. Conclusions: We identified candidate genes that showed highly dynamic changes in expression during the calyx abscission process. These genes are potential targets for future functional characterization and should be valuable for exploration of the mechanisms of calyx abscission, and eventually for developing methods based on small molecule application to induce calyx abscission in fruit production.

分类号：

  • 相关文献

[1]Transcriptome profiling reveals differentially expressed genes associated with wizened flower bud formation in Chinese pear (Pyrus bretschneideri Rehd.). Liu, Ya,Zhang, Hu Ping,Gu, Chao,Tao, Shu Tian,Qi, Kai Jie,Zhang, Shao Ling,Wang, Dong Sheng,Guo, Xian Ping.

[2]Development of a novel and efficient strategy for practical identification of Pyrus spp (Rosaceae) cultivars using RAPD fingerprints. Lin, J.,Chang, Y. H.,Wang, X. C.,Fang, J. G.,Wang, X. C.,Fang, J. G.. 2011

[3]Identification and expression patterns of alcohol dehydrogenase genes involving in ester volatile biosynthesis in pear fruit. Qin Gai-hua,Qi Yong-jie,Gao Zheng-hui,Pan Hai-fa,Xu Yi-liu,Qin Gai-hua,Qi Xiao-xiao,Qi Yong-jie,Gao Zheng-hui,Pan Hai-fa,Xu Yi-liu,Qi Xiao-xiao. 2017

[4]Dynamics of Oxine-Copper in Pears and Soil by High-Performance Liquid Chromatography. Li, Zeng-Mei,Deng, Li-Gang,Zhang, Shu-Qiu,Liang, Jing-Yun,Guo, Chang-Ying,Dong, Yan-Jie,Zhao, Shan-Cang,Li, Zeng-Mei,Deng, Li-Gang,Zhang, Shu-Qiu,Liang, Jing-Yun,Guo, Chang-Ying,Dong, Yan-Jie,Zhao, Shan-Cang,Wang, Yong-Qin. 2016

[5]Molecular cloning and gene expression differences of the anthocyanin biosynthesis-related genes in the red/green skin color mutant of pear (Pyrus communis L.). Yang, Ya-nan,Zhao, Guang,Zhang, Shao-ling,Gu, Chao,Wu, Jun,Yue, Wen-quan. 2013

[6]Characteristic Wavelengths Selection of Soluble Solids Content of Pear Based on NIR Spectral and LS-SVM. Fan Shu-xiang,Zhao Chun-jiang,Fan Shu-xiang,Huang Wen-qian,Li Jiang-bo,Zhao Chun-jiang,Zhang Bao-hua. 2014

[7]Studies on Correlation between Flowering and Maturing Stage in Pears. Li, X. G.,Yang, Q. S.,Lin, J.,Wang, Zh. H.,Chang, Y. H.. 2011

[8]Abscisic acid is involved in the water stress-induced betaine accumulation in pear leaves. Gao, XP,Pan, QH,Li, MJ,Zhang, LY,Wang, XF,Shen, YY,Lu, YF,Chen, SW,Liang, Z,Zhang, DP. 2004

[9]Calcium treatments promote the aroma volatiles emission of pear (Pyrus ussuriensis 'Nanguoli') fruit during post-harvest ripening process. Wei, Shuwei,Qin, Gaihua,Zhang, Huping,Tao, Shutian,Wu, Jun,Zhang, Shaoling,Wei, Shuwei,Wang, Shaomin,Qin, Gaihua.

[10]Evolution of the Aroma Volatiles of Pear Fruits Supplemented with Fatty Acid Metabolic Precursors. Qin, Gaihua,Tao, Shutian,Zhang, Huping,Huang, Wenjiang,Wu, Juyou,Zhang, Shaoling,Qin, Gaihua,Qin, Gaihua,Xu, Yiliu.

[11]Morphological, cytological and fertility consequences of a spontaneous tetraploid of the diploid pear (Pyrus pyrifolia Nakai) cultivar 'Cuiguan'. Wang, Xiaoqing,Shi, Chunhui,Zhang, Xueying,Duan, Ke,Luo, Jun,Wang, Xiaoqing,Shi, Chunhui,Zhang, Xueying,Duan, Ke,Luo, Jun,Wang, Haihai.

[12]Prediction of Soluble Solids Content and Firmness of Pears Using Hyperspectral Reflectance Imaging. Fan, Shuxiang,Huang, Wenqian,Guo, Zhiming,Zhang, Baohua,Zhao, Chunjiang,Fan, Shuxiang,Zhao, Chunjiang.

[13]A comparative study for the quantitative determination of soluble solids content, pH and firmness of pears by Vis/NIR spectroscopy. Li, Jiangbo,Huang, Wenqian,Zhao, Chunjiang,Zhang, Baohua.

[14]Characterization of Colletotrichum fructicola, a new causal agent of leaf black spot disease of sandy pear (Pyrus pyrifolia). Hong, N.,Wang, GuoPing,Zhang, P. F.,Zhai, L. F.,Zhang, X. K.,Hong, N.,Xu, WenXing,Wang, GuoPing,Zhang, P. F.,Zhai, L. F.,Zhang, X. K.,Hong, N.,Xu, WenXing,Wang, GuoPing,Huang, X. Z..

[15]Efficacy of virus elimination from in vitro-cultured sand pear (Pyrus pyrifolia) by chemotherapy combined with thermotherapy. Hu, G. J.,Hong, N.,Wang, L. P.,Wang, G. P.,Hong, N.,Wang, G. P.,Hu, H. J.. 2012

[16]Native Honey Bees Outperform Adventive Honey Bees in Increasing Pyrus bretschneideri (Rosales: Rosaceae) Pollination. Gemeda, Tolera Kumsa,Yang, Huipeng,Huang, Jiaxing,Wu, Jie,Shao, Youquan,Wu, Wenqin. 2017

[17]Risk assessment and ranking of pesticide residues in Chinese pears. Li Zhi-xia,Nie Ji-yun,Yan Zhen,Xu Guo-feng,Kuang Li-xue,Pan Li-gang,Pan Li-gang,Xie Han-zhong,Wang Cheng,Liu Chuan-de,Zhao Xu-bo,Guo Yong-ze. 2015

[18]Synthesis of Carbon Dots from Pear Juice for Fluorescence Detection of Cu2+ Ion in Water. Li, Defang,Zhao, Lining,Gong, Haoru. 2018

[19]Effects of 1-MCP on chlorophyll degradation pathway-associated genes expression and chloroplast ultrastructure during the peel yellowing of Chinese pear fruits in storage. Cheng, Yudou,Dong, Yu,Yan, Hongbo,Ge, Wenya,Guan, Junfeng,Liu, Liqin,Zhang, Yingying,Cheng, Yudou,Guan, Junfeng,Dong, Yu,Shen, Chengguo.

[20]Cloning, localization and expression analysis of two fw2.2-like genes in small- and large-fruited pear species. Tian Jia,Zeng Bin,Li Jiang,Luo Shu-ping,Li Xiu-gen,Wu Bin,Wu Bin.

作者其他论文 更多>>

微信小程序