Genome-wide transcriptional analysis of two soybean genotypes under dehydration and rehydration conditions

文献类型： 外文期刊

第一作者： Chen, Li M.

作者： Chen, Li M.;Zhou, Xin A.;Zhou, Rong;Wang, Cheng;Sha, Ai H.;Shan, Zhi H.;Zhang, Chan J.;Qiu, De Z.;Yang, Zhong L.;Chen, Shui L.;Chen, Li M.;Zhou, Xin A.;Zhou, Rong;Wang, Cheng;Sha, Ai H.;Shan, Zhi H.;Zhang, Chan J.;Qiu, De Z.;Yang, Zhong L.;Chen, Shui L.;Chen, Li M.;Li, Wen B.;Chang, Wei;Chen, Li M.;Li, Wen B.;Chang, Wei

作者机构：

关键词： Soybean;Dehydration;Digital gene expression tag profile;Rehydration;Differentially expressed genes;Quantitative RT-PCR;Transcription factors;Protein kinases;Regulatory proteins

期刊名称：BMC GENOMICS （ 影响因子：3.969； 五年影响因子：4.478 ）

ISSN： 1471-2164

年卷期： 2013 年 14 卷

页码：

收录情况： SCI

摘要： Background: Soybean is an important crop that provides valuable proteins and oils for human use. Because soybean growth and development is extremely sensitive to water deficit, quality and crop yields are severely impacted by drought stress. In the face of limited water resources, drought-responsive genes are therefore of interest. Identification and analysis of dehydration-and rehydration-inducible differentially expressed genes (DEGs) would not only aid elucidation of molecular mechanisms of stress response, but also enable improvement of crop stress tolerance via gene transfer. Using Digital Gene Expression Tag profiling (DGE), a new technique based on Illumina sequencing, we analyzed expression profiles between two soybean genotypes to identify drought-responsive genes. Results: Two soybean genotypes-drought-tolerant Jindou21 and drought-sensitive Zhongdou33-were subjected to dehydration and rehydration conditions. For analysis of DEGs under dehydration conditions, 20 cDNA libraries were generated from roots and leaves at two different time points under well-watered and dehydration conditions. We also generated eight libraries for analysis under rehydration conditions. Sequencing of the 28 libraries produced 25,000-33,000 unambiguous tags, which were mapped to reference sequences for annotation of expressed genes. Many genes exhibited significant expression differences among the libraries. DEGs in the drought-tolerant genotype were identified by comparison of DEGs among treatments and genotypes. In Jindou21, 518 and 614 genes were differentially expressed under dehydration in leaves and roots, respectively, with 24 identified both in leaves and roots. The main functional categories enriched in these DEGs were metabolic process, response to stresses, plant hormone signal transduction, protein processing, and plant-pathogen interaction pathway; the associated genes primarily encoded transcription factors, protein kinases, and other regulatory proteins. The seven most significantly expressed (vertical bar log(2) ratio vertical bar >= 8) genes-Glyma15g03920, Glyma05g02470, Glyma15g15010, Glyma05g09070, Glyma06g35630, Glyma08g12590, and Glyma11g16000-are more likely to determine drought stress tolerance. The expression patterns of eight randomly-selected genes were confirmed by quantitative RT-PCR; the results of QRT-PCR analysis agreed with transcriptional profile data for 96 out of 128 (75%) data points. Conclusions: Many soybean genes were differentially expressed between drought-tolerant and drought-sensitive genotypes. Based on GO functional annotation and pathway enrichment analysis, some of these genes encoded transcription factors, protein kinases, and other regulatory proteins. The seven most significant DEGs are candidates for improving soybean drought tolerance. These findings will be helpful for analysis and elucidation of molecular mechanisms of drought tolerance; they also provide a basis for cultivating new varieties of drought-tolerant soybean.

分类号：

  • 相关文献

[1]Global Gene Expression Analysis Reveals Crosstalk between Response Mechanisms to Cold and Drought Stresses in Cassava Seedlings. Li, Shuxia,Yu, Xiaoling,Ruan, Mengbin,Li, Wenbin,Peng, Ming,Yu, Xiang,Yu, Xiang,Cheng, Zhihao,Yu, Xiang. 2017

[2]An organ-specific role for ethylene in rose petal expansion during dehydration and rehydration. Liu, Daofeng,Liu, Xiaojing,Meng, Yonglu,Sun, Cuihui,Tang, Hongshu,Jiang, Yudong,Khan, Muhammad Ali,Ma, Nan,Gao, Junping,Liu, Daofeng,Liu, Xiaojing,Xue, Jingqi.

[3]Reduced Glutathione Mediates Phone-Ultrastrcture, Kinome and Transportome in Chromium-Induced Brassica napus L.. Gill, Rafaqat A.,Ali, Basharat,Yang, Su,Islam, Faisal,Gill, Muhammad Bilal,Mwamba, Theodore M.,Ali, Skhawat,Zhou, Weijun,Gill, Rafaqat A.,Ali, Basharat,Yang, Su,Islam, Faisal,Gill, Muhammad Bilal,Mwamba, Theodore M.,Ali, Skhawat,Zhou, Weijun,Gill, Rafaqat A.,Mao, Bizeng,Ali, Basharat,Tong, Chaobo,Liu, Shengyi. 2017

[4]Differential expression profiling of the early response to Ustilaginoidea virens between false smut resistant and susceptible rice varieties. Han, Yanqing,Zhang, Kang,Yang, Jun,Zhang, Nan,Fang, Anfei,Zhang, Yong,Sun, Wenxian,Han, Yanqing,Zhang, Kang,Yang, Jun,Zhang, Nan,Fang, Anfei,Zhang, Yong,Sun, Wenxian,Liu, Yongfeng,Chen, Zhiyi,Hsiang, Tom. 2015

[5]Molecular cloning, expression analysis, and subcellular localization of FLOWERING LOCUS T (FT) in carrot (Daucus carota L.). Zhan, Zongxiang,Zhang, Chunsha,Zhang, Hongwei,Li, Xiaojie,Wen, Changlong,Liang, Yi,Zhan, Zongxiang,Zhan, Zongxiang. 2017

[6]Transcript analysis and expression profiling of three heat shock protein 70 genes in the ectoparasitoid Habrobracon hebetor (Hymenoptera: Braconidae). Chen, Hao-Liang,Zhangl, Hong-Yu,Chen, Hao-Liang,Chen, Hao-Liang,Throne, James E.,Chen, Hao-Liang,Zhu, Kun Yan. 2014

[7]Global characterization of microRNAs in Trichomonas gallinae. Xu, Min-Jun,Qiu, Shen-Ben,Fu, Jing-Hua,Zhu, Xing-Quan,Nisbet, Alasdair J.,Qiu, Shen-Ben,Fu, Jing-Hua,Shao, Chang-Chun. 2014

[8]Molecular cloning, expression analysis and subcellular localization of a Transparent Testa 12 ortholog in brown cotton (Gossypium hirsutum L.). Gao, Jun-Shan,Wu, Nan,Shen, Zhi-Lin,Qian, Sen-He,Guo, Ning,Sun, Xu,Cai, Yong-Ping,Lin, Yi,Lv, Kai.

[9]Molecular cloning, expression analysis and subcellular localization of LEAFY in carrot (Daucus carota L.). Zhang, Chunsha,Liang, Yi,Zhang, Hongwei,Zhan, Zongxiang,Zhan, Zongxiang.

[10]Effects of Exogenous Chitosan on Physiological Characteristics of Potato Seedlings Under Drought Stress and Rehydration. Jiao, Zhili,Li, Yong,Lu, Dianqiu,Li, Juanjuan,Xu, Xiaoyan,Wang, Jingying,Li, Hui. 2012

[11]Antitranspirant compounds alleviate the mild-desiccation-induced reduction of vase life in cut roses. Fanourakis, Dimitrios,Kambourakis, Emmanouil,Ligoxigakis, Eleftherios K.,Fanourakis, Dimitrios,Papadimitriou, Michael,Strataridaki, Argiro,Giday, Habtamu,Ottosen, Carl-Otto,Li, Tao,Li, Tao,Bouranis, Dimitrios,Fiorani, Fabio,Heuvelink, Ep.

[12]RhMKK9, a rose MAP KINASE KINASE gene, is involved in rehydration-triggered ethylene production in rose gynoecia. Chen, Jiwei,Zhang, Qian,Feng, Ming,Li, Yang,Meng, Yonglu,Zhang, Yi,Liu, Guoqin,Ma, Zhimin,Gao, Junping,Ma, Nan,Chen, Jiwei,Zhang, Qian,Feng, Ming,Li, Yang,Zhang, Yi,Liu, Guoqin,Ma, Zhimin,Gao, Junping,Ma, Nan,Wang, Qigang,Meng, Yonglu,Wu, Hongzhi. 2017

[13]Influence of pre-drying treatments on physicochemical and organoleptic properties of explosion puff dried jackfruit chips. Yi, Jianyong,Zhou, Linyan,Bi, Jinfeng,Chen, Qinqin,Liu, Xuan,Wu, Xinye.

[14]Physiological changes and differential gene expression of tea plant under dehydration and rehydration conditions. Liu, Sheng-Chuan,Yao, Ming-Zhe,Ma, Chun-Lei,Jin, Ji-Qiang,Ma, Jian-Qiang,Li, Chun-Fang,Chen, Liang,Liu, Sheng-Chuan.

[15]Developing Novel Combination Drying Method for Jackfruit Bulb Chips: Instant Controlled Pressure Drop (DIC)-Assisted Freeze Drying. Yi, Jianyong,Wang, Ping,Bi, Jinfeng,Liu, Xuan,Wu, Xinye,Wang, Ping,Zhong, Yaoguang.

[16]Effects of superabsorbent polymer on the physiological characteristics of wheat under drought stress and rehydration. Yang, Yonghui,Wu, Jicheng,Wu, Pute. 2011

[17]Effect of microwave power on kinetics and characteristics of microwave vacuum-dried longan (Dimocarpus longan Lour.) pulp. Su, Dongxiao,Zhang, Mingwei,Wei, Zhencheng,Tang, Xiaojun,Zhang, Ruifen,Liu, Lei,Deng, Yuanyuan,Su, Dongxiao,Zhang, Mingwei.

[18]Highly efficient plant regeneration through somatic embryogenesis in 20 elite commercial cotton (Gossypium hirsutum L.) cultivars. Baohong Zhang,Qinglian Wang,Fang Liu,Kunbo Wang,Taylor P. Frazier. 2009

[19]microRNA/microRNA* complementarity is important for the regulation pattern of NFYA5 by miR169 under dehydration shock in Arabidopsis. Du, Qingguo,Gao, Wei,Sun, Suzhen,Li, Wen-Xue,Zhao, Meng. 2017

[20]A low drying temperature benefits the quality of dehydrated thyme. Zhang, Chao. 2015

作者其他论文 更多>>

微信小程序