Outbreak of Porcine Epidemic Diarrhea in Piglets in Gansu Province, China

文献类型： 外文期刊

第一作者： Wang, Hui

作者： Wang, Hui;Xia, Xinchao;Liu, Yongming;Wang, Shengyi;Qi, Zhiming;Liu, Shixiang;Wang, Haijun;Liu, Zhiqi;Niu, Xu;Liu, Shanshan

作者机构：

关键词： porcine epidemic diarrhea;PEDV;Real-time RT-PCR;epidemiology;Gansu

期刊名称：ACTA SCIENTIAE VETERINARIAE （ 影响因子：0.337； 五年影响因子：0.41 ）

ISSN： 1678-0345

年卷期： 2013 年 41 卷

页码：

收录情况： SCI

摘要： Background: Porcine epidemic diarrhea virus (PEDV) is a member of the genus Coronavirus and family Coronaviridae. The virus is the aetiological agent of porcine epidemic diarrhea (PED), and detected for the first time in 1978 in England. PED causes acute enteritis and severe diarrheal disease with high mortality, particularly in suckling piglets; massive economic losses in many locations resulted. The severe acute diarrhea outbreak associated with high morbidity and mortality. By now, PEDV cases confirmed by laboratory testing have been reported from a lot of countries (southern China, Thailand, Vietnam, Korea and Japan). Gansu is located in the northwest of China; and few case of PEDV infection has been detected. And previous research has shown that the immunity induced by the commercial Korean vaccine do not provide cross-protection to the recent Chinese PEDV strain. So the virus may as a variant PEDV emerging in Gansu. The study was aimed at detecting the presence of piglets positive to PEDV in Gansu province of China. Materials, Methods & Results: A total of 120 samples were collected from piglets from different farms in Gansu province, China from March 2011 to December 2012. The clinical symptoms and anatomic pathological changes of diarrheal piglets were observed. The total RNA was extracted from intestinal contents of diarrheal suckling piglets with a commercial High Pure Viral RNA Kit. Real-time RT-PCR was used to investigate enteropathogen. The reverse transcription was prepared in total of 25 mu L volume according to the manufacturers' instructions with the following component volumes per well: 8 mu L total RNA, nuclease-free water 2.5 mu L, RT-PCR reaction liquid 12.5 mu L, multiplex RT-PCR enzyme mix 1.0 mu L, and fluorescence probe 1.0 mu L. The reaction was performed at 48 degrees C 10 min, 95 degrees C 10 min, 95 degrees C 15 s, 60 degrees C 45 s. Real-time PCR was performed using ABI PRISM 7500 real-time PCR machine. Samples with threshold cycles (Cts) of 35 cycles were considered positive, and those with Cts < 35 were considered negative. Quality control for the PCR reaction included nuclease-free water as negative amplification control in addition to a positive amplification control provided by the manufacturer. The diarrheal piglets showed severe watery diarrhea, dehydration with milk curd vomitus, mild hemorrhage, undigested curdled milk in the stomach, and thin-walled intestines with severe mucosal atrophy and foamy fluid. The Real-time RT-PCR test identified 87 positive piglets - 18 in Jiayuguan (18% of all tested piglets), 12 in Wuwei (10% of all tested piglets), 11 in Shuangta (9.2% of all tested piglets), 30 in Jingning (25% of all tested piglets) and 16 in Tianshui (13.3% of all tested piglets). Discussion: Relatively higher number of positive piglets in the Gansu Province, Northwest China indicates that this is the region where PEDV has entered the country. And the outbreak of PEDV in Gansu caused severe diarrheal disease in piglets; heavy economic losses in many farms resulted (mortality was 60-85%). Given that the infection has been so far reported only from southern China, our study implies the potential of PEDV to cover new territories. Coronavirus is a significant risk pathogen in humans and animals. Animal Coronavirus is a very serious risk to sustained and healthy development of animal husbandry. Therefore, effective biosecurity control and strict sanitary measures are key components of PED prevention and control. Meanwhile the potential mechanism of PEDV interacts with the host antiviral innate system and the molecular mechanisms that regulate the host innate antiviral immune responses should be further studied.

分类号：

  • 相关文献

[1]Protecting Local Breeds of Livestock in NW China. Lang Xia,Wang Cailian,Squires, Victor. 2010

[2]Redesigning Livestock Systems to Improve Household Income and Reduce Stocking Rates in China's Western Grasslands. Michalk, D. L.,Hua Limin,Wu Jianping,Kemp, David,Takahashi, Taro,Jones, Randall,Nan Zhibiao,Xu Zhu,Han Guodong. 2010

[3]First Molecular Detection of Piroplasm Infection in Pet Dogs from Gansu, China. Niu, Qingli,Yang, Jifei,Liu, Zhijie,Gao, Shandian,Pan, Yuping,Guan, Guiquan,Chu, Yuefeng,Liu, Guangyuan,Luo, Jianxun,Yin, Hong,Yin, Hong. 2017

[4]Interspecific complementary and competitive interactions between intercropped maize and faba bean. Li, L,Yang, SC,Li, XL,Zhang, FS,Christie, P.

[5]An efficient method of noroviruses recovery from oysters and clams. Zhou Deqing,Ma Liping,Zhao Feng,Yao Lin,Li Xinguang,Ma Liping,Su Laijin,Li Xinguang. 2013

[6]Differentially expressed genes under cold acclimation in Physcomitrella patens. Sun, Ming-Ming,Li, Lin-Hui,Xie, Hua,Ma, Rong-Cai,He, Yi-Kun. 2007

[7]Tissue distribution and expression of signaling lymphocyte activation molecule receptor to peste des petits ruminant virus in goats detected by real-time PCR. Meng, Xuelian,Dou, Yongxi,Zhai, Junjun,Zhang, Hairui,Yan, Fengchao,Shi, Xiaoni,Luo, Xuelong,Li, Hui,Cai, Xuepeng. 2011

[8]Development of a one-step real-time RT-PCR assay using a minor-groove-binding probe for the detection of duck Tembusu virus. Yun, Tao,Ni, Zheng,Hua, Jionggang,Ye, Weicheng,Chen, Liu,Zhang, Shuai,Zhang, Yan,Zhang, Cun,Zhang, Shuai. 2012

[9]A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification. Kang, Kang,Luo, Jun,Kang, Kang,Zhong, Jiasheng,Zhang, Limin,Gou, Deming,Yang, Keli,Tian, Yongxiang,Zhai, Jianxin,Zhang, Li,Song, Changxu,Gou, Christine Yuan. 2014

[10]Pathogenesis of a genotype C strain of bovine parainfluenza virus type 3 infection in albino guinea pigs. Shi, Hong-Fei,Zhu, Yuan-Mao,Dong, Xiu-Mei,Cai, Hong,Ma, Lei,Wang, Shu,Yan, Hao,Wang, Xue-Zhi,Xue, Fei.

[11]Relation Between the Viral Load Accumulation of Southern Rice Black-Streaked Dwarf Virus and the Different Developmental Stages of Sogatella furcifera (Hemiptera: Delphacidae). Hou, Mao-Lin,Liu, Yu-Di.

[12]Development of a real-time RT-PCR method for the detection of newly emerged highly pathogenic H7N9 influenza viruses. Wang Xiu-rong,Gu Lin-lin,Shi Jian-zhong,Xu Hai-feng,Zhang Ying,Zeng Xian-ying,Deng Guo-hua,Li Cheng-jun,Chen Hua-lan. 2017

[13]The survey of porcine teschoviruses in field samples in China with a universal rapid probe real-time RT-PCR assay. Zhang, Chaofan,Wang, Ming,Zhang, Chaofan,Hu, Feng,Qiu, Zheng,Cui, Shangjin,Wang, Zhongtian,Liu, Yebing,Zhou, Shun.

[14]Screening of valid reference genes for real-time RT-PCR data normalization in Hevea brasiliensis and expression validation of a sucrose transporter gene HbSUT3. Li, Heping,Qin, Yunxia,Xiao, Xiaohu,Tang, Chaorong,Li, Heping,Qin, Yunxia,Xiao, Xiaohu,Tang, Chaorong,Li, Heping,Xiao, Xiaohu. 2011

[15]Evaluation of a real-time RT-PCR assay using minor groove binding probe for specific detection of Chinese wild-type classical swine fever virus. Wen, Guoyuan,Yang, Jun,Luo, Qingping,Liao, Yonghong,Zhang, Rongrong,Wang, Hongling,Ai, Diyun,Luo, Ling,Shao, Huabin,Zhang, Tao,Wen, Guoyuan,Hu, Zhibin,Song, Nianhua,Zhang, Tao. 2011

[16]Plus- and minus-stranded foot-and-mouth disease virus RNA quantified simultaneously using a novel real-time RT-PCR. Gu, Chaojiang,Zheng, Congyi,Shi, Lili,Zhang, Qian,Li, Yong,Lu, Bin,Xiong, Yi,Qu, Sanpu,Shao, Junjun,Chang, Huiyun. 2007

[17]The presence of Genogroup II Norovirus in Retail Shellfish from Seven Coastal Cities in China. Ma, Li-ping,Zhao, Feng,Yao, Lin,Li, Xin-guang,Zhou, De-qing,Zhang, Rui-ling,Ma, Li-ping.

[18]Development of a real-time reverse transcription loop-mediated isothermal amplification method for the rapid detection of porcine epidemic diarrhea virus. Yu, Xuewu,Shi, Lin,Lv, Xiaoping,Zheng, Shimin,Yu, Xuewu,Shi, Lin,Yao, Wei,Cao, Minghui,Yu, Hanxun,Wang, Xiurong,Wang, Xiurong. 2015

[19]Detection and phylogenetic analyses of spike genes in porcine epidemic diarrhea virus strains circulating in China in 2016-2017. Zhang, Qiaoling,Liu, Xinsheng,Fang, Yuzhen,Zhou, Peng,Wang, Yonglu,Zhang, Yongguang,Zhang, Qiaoling,Liu, Xinsheng,Fang, Yuzhen,Zhou, Peng,Wang, Yonglu,Zhang, Yongguang. 2017

[20]Detection and phylogenetic analysis of porcine epidemic diarrhea virus in central China based on the ORF3 gene and the S1 gene. Su, Yunfang,Ji, Pengchao,Liu, Yunchao,Chen, Yumei,Xing, Guangxu,Liu, Chang,Deng, Ruiguang,Zhang, Gaiping,Zhao, Baolei,Jiang, Dawei,Song, Yapeng,Wang, Guoqiang,Li, Dongliang,Zhang, Gaiping,Zhang, Gaiping. 2016

作者其他论文 更多>>

微信小程序