Identification and characterization of new major sperm protein genes from Oesophagostomum dentatum and Oesophagostomum quadrispinulatum from pigs in China

文献类型： 外文期刊

第一作者： Lin, Rui-Qing

作者： Lin, Rui-Qing;Zhou, Dong-Hui;Huang, Si-Yang;Zhang, Yuan;Song, Hui-Qun;Zhu, Xing-Quan;Lin, Rui-Qing;Zhang, Yuan;Song, Hui-Qun;Weng, Ya-Biao;Zou, Feng-Cai;Zhu, Xing-Quan;Zhu, Xing-Quan

作者机构：

关键词： Major sperm protein (MSP) gene;Nodule worm;Oesophagostomum dentatum;Oesophagostomum quadrispinulatum;Sequence variation

期刊名称：EXPERIMENTAL PARASITOLOGY （ 影响因子：2.011； 五年影响因子：2.132 ）

ISSN： 0014-4894

年卷期： 2013 年 133 卷 2 期

页码：

收录情况： SCI

摘要： The present study identified and characterized new major sperm protein (MSP) genes from the two nodule worms Oesophagostomum dentatum and Oesophagostomum quadrispinulatum collected from pigs in China. Total genomic DNA was extracted individually from 10 male nematode samples representing O. dentatum, and 4 male nematode samples representing O. quadrispinulatum. A pair of primers (OMSP1F/MSP1R) was designed based on the MSP gene sequences of Ascaris suum and O. dentatum available in GenBank, and used to amplify the MSP genes from the two porcine nodule worms. The PCR products were purified and subsequently cloned into pGEM-T Easy vector. Recombinants were identified by PCR and sequenced. Sequence analysis revealed that there were two different types of MSP sequences in O. dentatum and O. quadrispinulatum, one contained intron, and the other did not. The lengths of the MSP sequences containing introns were 433 bp or 439 bp in O. dentatum, and 436 bp, 439 bp or 446 bp in O. quadrispinulatum, containing 1 or 2 introns. Five and three new members of the MSP multigene family were identified in O. dentatum and O. quadrispinulatum in this study, respectively. The MSP sequences without introns were 381 bp in length, and can be deduced into 126 amino acids. The sequences of MSP genes containing introns seem to be more conserved than those without introns. The identities of deduced amino acid sequences of the MSP genes containing introns were 96.0-100% within and between the two nodule worms, and were 81.1-93.7% compared with other published MSP sequences of the representative nematodes. The present study identified new MSP genes with introns from O. dentatum and O. quadrispinulatum for the first time. The identification and characterization of newly described MSP genes from O. dentatum and O. quadrispinulatum have implications for further studies of molecular biology and reproduction control of Oesophagostomum spp. (C) 2012 Elsevier Inc. All rights reserved.

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