Enhanced protective immune response to PCV2 subunit vaccine by co-administration of recombinant porcine IFN-gamma in mice

文献类型: 外文期刊

第一作者: Wang, Yi-Ping

作者: Wang, Yi-Ping;Liu, Dan;Guo, Long-Jun;Tang, Qing-Hai;Wei, Yan-Wu;Wu, Hong-Li;Liu, Jian-Bo;Li, Sheng-Bin;Huang, Li-Ping;Liu, Chang-Ming

作者机构:

关键词: Porcine circovirus type 2 (PCV2);Capsid (Cap) protein;Porcine interferon-gamma (PoIFN-gamma);Recombinant baculoviruses;Immune response

期刊名称:VACCINE ( 影响因子:3.641; 五年影响因子:3.816 )

ISSN: 0264-410X

年卷期: 2013 年 31 卷 5 期

页码:

收录情况: SCI

摘要: The capsid (Cap) protein of PCV2 is the major immunogenic protein that is crucial to induce PCV2-specific neutralizing antibodies and protective immunity; thus, it is a suitable target antigen for the research and development of genetically engineered vaccines against PCV2 infection. IFN-gamma has exhibited potential efficacy as an immune adjuvant that enhances the immunogenicity of certain vaccines in experimental animal models. In this study, three recombinant proteins: PCV2-Cap protein, porcine IFN-gamma (PoIFN-gamma), and the fusion protein (Cap-PoIFN-gamma) of PCV2-Cap protein and PoIFN-gamma were respectively expressed in the baculovirus system, and analyzed by Western blot and indirect ELISA. Additionally, we evaluated the enhancement of the protective immune response to the Cap protein-based PCV2 subunit vaccine elicited by co-administration of PoIFN-gamma in mice. Vaccination of mice with the PCV2-Cap + PoIFN-gamma vaccine elicited significantly higher levels of PCV2-specific IPMA antibodies, neutralizing antibodies, and lymphocyte proliferative responses compared to the Cap-PoIFN-gamma vaccine, the PCV2-Cap vaccine, and LG-strain. Following virulent PCV2 challenge, no viraemia was detected in all immunized groups, and the viral loads in lungs of the PCV2-Cap + PoIFN-gamma group were significantly lower compared to the Cap-PoIFN-gamma group, the LG-strain group, and the mock group, but slightly lower compared to the PCV2-Cap group. These findings suggested that PoIFN-gamma substantially enhanced the protective immune response to the Cap protein-based PCV2 subunit vaccine, and that the PCV2-Cap + PoIFN-gamma subunit vaccine potentially serves as an attractive candidate vaccine for the prevention and control of PCV2-associated diseases. (C) 2012 Elsevier Ltd. All rights reserved.

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