Integration and Characterization of T-DNA Insertion in Upland Cotton

文献类型： 外文期刊

第一作者： Xiaojie YANG

作者： Xiaojie YANG;Fuguang LI;Xueyan ZHANG;Kun LIU;Qianhua WANG;Chaojun ZHANG;Chuanliang LIU;Wei ZHU;Guofang SHAN;Chee-Kok CHIN;Weiping FANG

作者机构：

关键词： cotton (Gossypium hirsutum L.);deletion of border;genetic transformation;transgene copy;vector integration

期刊名称：CZECH JOURNAL OF GENETICS AND PLANT BREEDING （ 影响因子：0.865； 五年影响因子：1.017 ）

ISSN： 1212-1975

年卷期： 2013 年 49 卷 2 期

页码：

收录情况： SCI

摘要： Copy numbers were evaluated by real-time quantitative PCR, and 149 junctions of T-DNA were isolated by thermal asymmetric interlaced PCR from 92 independent transgenic cotton lines transformed by Agrobacterium tumefaciens strain LBA4404. Real-time quantitative PCR results showed that 46% had integration of one or two T-DNA copies, 54% had three or more copies. Among 63 amplified products at LB junctions, 51% showed cotransformation of the vector backbone, 30% retained a portion of LB ranging from 3 to 23 bp, and 19% showed deletions ranging from 1 to 148 bp from the LB inner end. In contrast, all of the cleavage sites were located in the inner region of RB. The distribution of T-DNA insertions in upland cotton genome included coding sequences, transposons, plastid-derived sequences and microsatellites.

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