Reversible immobilization of K. fragilis beta-galactosidase onto magnetic polyethylenimine-grafted nanospheres for synthesis of galacto-oligosaccharide

文献类型： 外文期刊

第一作者： Liu, Jian-Fu

作者： Liu, Jian-Fu;Liu, Jian-Fu;Liu, Hong;Tan, Bin;Chen, Yong-Hong;Yang, Rui-Jin;Yang, Rui-Jin

作者机构：

关键词： Kluyveromyces fragilis beta-galactosidase;Reversible immobilization;Magnetic polyethylenimine-grafted;nanospheres;Galacto-oligosaccharide

期刊名称：JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC （ 影响因子：2.269； 五年影响因子：2.328 ）

ISSN： 1381-1177

年卷期： 2012 年 82 卷

页码：

收录情况： SCI

摘要： A nanosized support for reversible immobilization of enzymes, magnetic poly(glycidyl methacrylate-ethylene glycol dimethacrylate-hydroxyethyl methacrylate) nanospheres grafted with polyethyleneimine, was prepared and characterized by scanning electron microscopy and vibrating sample magnetometry. The novel support displayed 3.2-fold higher adsorption capacity for Kluyveromyces fragilis beta-galactosidase than commercial ion exchange resin DEAE-Sepharose, the maximum amount of the enzyme adsorbed on the support reached 86.7 mg/g with enzyme activity recovery of 92.5%. The adsorbed K. fragilis beta-galactosidase showed high catalytic activity and operational stability for synthesis of galacto-oligosaccharide (GOS), 4.5 kg of GOS were produced per gram of adsorbed enzyme from lactose during 15 consecutive batch reactions by reuse of the same biocatalyst, and the immobilized biocatalyst retained about 84.6% of its initial activity after 15 cycles of batch operation. The support could be regenerated and reused, remaining 93.0% of its original adsorption capacity at the end of the twentieth adsorption-desorption cycle. Crown Copyright (C) 2012 Published by Elsevier B.V. All rights reserved,

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