Covalent immobilization of Kluyveromyces fragilis beta-galactosidase on magnetic nanosized epoxy support for synthesis of galacto-oligosaccharide

文献类型： 外文期刊

第一作者： Liu, Jianfu

作者： Liu, Jianfu;Qin, Yan;Liu, Hong;Liu, Jianfu;Tan, Bin;Zhou, Fenjuan;Yang, Ruijin;Yang, Ruijin

作者机构：

关键词： Kluyveromyces fragilis beta-galactosidase;Magnetic nanosized epoxy support;Covalent immobilization;Galacto-oligosaccharide

期刊名称：BIOPROCESS AND BIOSYSTEMS ENGINEERING （ 影响因子：3.21； 五年影响因子：2.97 ）

ISSN： 1615-7591

年卷期： 2012 年 35 卷 8 期

页码：

收录情况： SCI

摘要： The novel magnetic nanobeads with epoxy groups on the surface were prepared from glycidyl methacrylate (GMA), ethylene glycol dimethacrylate (EGDMA) and hydroxyethyl methacrylate (HEMA) via emulsifier-free emulsion polymerisation, and they were characterized by scanning electron microscopy and vibrating sample magnetometer. The magnetic poly(GMA-EDGMA-HEMA) nanobeads were used as support for covalent immobilization of Kluyveromyces fragilis beta-galactosidase, the maximum amount enzyme attached onto the support was 145.6 mg/g with activity recovery of 72.6%. The loading capacity of this novel support for K. fragilis beta-galactosidase was improved 2.6-folds compared with Eupergit(A (R)) C (commercial epoxy support). The immobilized K. fragilis beta-galactosidase exhibited high catalytic activity for the reaction of galacto-oligosaccharide (GOS) synthesis, and a total of 2,240 g GOS were produced per gram of immobilized enzyme during consecutive batch reaction of 10 times. The immobilized biocatalyst retained 81.5% of its original activity after 10 reaction cycles.

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[1]Reversible immobilization of K. fragilis beta-galactosidase onto magnetic polyethylenimine-grafted nanospheres for synthesis of galacto-oligosaccharide. Liu, Jian-Fu,Liu, Jian-Fu,Liu, Hong,Tan, Bin,Chen, Yong-Hong,Yang, Rui-Jin,Yang, Rui-Jin. 2012

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