Purification of Transglutaminase from High Salt Culture by Gel and Cation Exchange Chromatography
文献类型: 外文期刊
第一作者: Zhang Li-Li
作者: Zhang Li-Li;Zhang Lan-Wei;Du Ming;Han Xue;Yi Hua-Xi;Zhang Ying-Chun;Ma Wei;Zhang Yan-He
作者机构:
关键词: Transglutaminase;Purification;Liquid chromatography/tandem mass spectrometry;Gel chromatography;Cation exchange chromatography
期刊名称:CHINESE JOURNAL OF ANALYTICAL CHEMISTRY ( 影响因子:1.134; 五年影响因子:0.909 )
ISSN: 0253-3820
年卷期: 2012 年 40 卷 8 期
页码:
收录情况: SCI
摘要: To purify the transglutaminase from high-salt medium, gel filtration combined with ion exchange chromatography method was employed. Two-step chromatographic conditions (flow rate and the sample volume) were optimized. The final sample volume and a flow rate for Gel chromatography were 6 mL and 0. 25 mL/min, respectively. Conditions for ion-exchange chromatography were a sample volume of 50 mL and flow rate of 3 mL/min. Enzyme was purified 4. 22-fold, the specific activity and the recovery rate were 17. 33 U/mg protein and 77. 5%, respectrively. A comparison between liquid chromatography/tandem mass spectrometric identification and the protein database was carried out and the results showed that the purified enzyme had high homology with AAN01353.
分类号:
- 相关文献
作者其他论文 更多>>
-
Rapid Identification of Inorganic Elements in Understory Soils in Different Regions of Guizhou Province by X-Ray Fluorescence Spectrometry
作者:HAN Xue;LIU Hai;WU Ming-Kai;HAN Xue;LIU Hai;WU Ming-Kai;LIU Jia-Wei
关键词:XRD; Understory soil; Inorganic element
-
Identification of candidate genes related to soluble sugar contents in soybean seeds using multiple genetic analyses
作者:Pan Wen-jing;Huang Shi-yu;Yu Jing-yao;Zhao Ying;Qu Ke-xin;Zhang Ze-xin;Yin Zhen-gong;Qi Hui-dong;Yu Guo-long;Xin Da-wei;Zhu Rong-sheng;Liu Chun-yan;Wu Xiao-xia;Jiang Hong-wei;Hu Zhen-bang;Chen Qing-shan;Qi Zhao-ming;Han Xue;Zuo Yu-hu;Zhang Yong
关键词:soybean soluble sugar contents; consensus QTL; meta-overview-collinearity integrated analysis; population validation; RNA-seq and candidate gene mining
-
Strip deep rotary tillage combined with controlled-release urea improves the grain yield and nitrogen use efficiency of maize in the North China Plain
作者:Han Yu-ling;Guo Dong;Ma Wei;Mehmood, Ali Noor;Zhao Ming;Zhou Bao-yuan;Guo Dong;Ge Jun-zhu;Li Xiang-ling
关键词:maize; strip deep rotary tillage; controlled-release urea; nitrogen accumulation; grain yield
-
Quantitative design of yield components to simulate yield formation for maize in China
作者:Hou Hai-peng;Ma Wei;Noor, Mehmood Ali;Li Cong-feng;Ding Zai-song;Zhao Ming;Tang Li-yuan
关键词:maize; yield performance parameters; high yield; yield prediction process; quantitative design
-
Innovation of the double-maize cropping system based on cultivar growing degree days for adapting to changing weather conditions in the North China Plain
作者:Wang Dan;Zhan Ming;Cao Cou-gui;Xia Fei;Wang Dan;Zhou Bao-yuan;Ma Wei;Zhao Ming;Wang Dan;Li Guo-rui;Meng Qing-feng
关键词:double-maize cropping system; weather conditions; grain yield; North China Plain
-
Post-silking nitrogen accumulation and remobilization are associated with green leaf persistence and plant density in maize
作者:Zhang Li-li;Qi Huai;Zhang Li-li;Fan Ye;Fu Jun;Yang Hai-long;Zhou Xiang-li;Hou Peng
关键词:maize; N remobilization; post-silking N uptake; stay green; plant density
-
Tomato Leaf Liriomyza Sativae Blanchard Pest Detection Based on Hyperspectral Technology
作者:Li Cui-ling;Jiang Kai;Ma Wei;Wang Xiu;Meng Zhi-jun;Zhao Xue-guan;Song Jian;Li Cui-ling;Jiang Kai;Ma Wei;Wang Xiu;Meng Zhi-jun;Zhao Xue-guan;Song Jian
关键词:Hyperspectral technology;Tomato;Pest;SAM;Red edge parameter;DA