Structure-Based High-Throughput Epitope Analysis of Hexon Proteins in B and C Species Human Adenoviruses (HAdVs)

文献类型： 外文期刊

第一作者： Yuan, Xiao-Hui

作者： Yuan, Xiao-Hui;Jin, Wen-Jing;Zhao, Bin-Bin;Chen, Cai-Feng;Yang, Jian;Liu, Jing-Jun;Zhang, Ding;Gong, Lu-Lu;He, You-Wen;Yuan, Xiao-Hui;Jin, Wen-Jing;Zhao, Bin-Bin;Chen, Cai-Feng;Yang, Jian;Liu, Jing-Jun;Zhang, Ding;Gong, Lu-Lu;He, You-Wen;Wang, Ying-Chen;Wang, Jing-Fei;Guo, Ying-Ying;He, You-Wen

作者机构：

期刊名称：PLOS ONE （ 影响因子：3.24； 五年影响因子：3.788 ）

ISSN： 1932-6203

年卷期： 2012 年 7 卷 3 期

页码：

收录情况： SCI

摘要： Human adenoviruses (HAdVs) are the etiologic agent of many human infectious diseases. The existence of at least 54 different serotypes of HAdVs has resulted in difficulties in clinical diagnosis. Acute respiratory tract disease (ARD) caused by some serotypes from B and C species is particularly serious. Hexon, the main coat protein of HAdV, contains the major serotype-specific B cell epitopes; however, few studies have addressed epitope mapping in most HAdV serotypes. In this study, we utilized a novel and rapid method for the modeling of homologous proteins based on the phylogenetic tree of protein families and built three-dimensional (3D) models of hexon proteins in B and C species HAdVs. Based on refined hexon structures, we used reverse evolutionary trace (RET) bioinformatics analysis combined with a specially designed hexon epitope screening algorithm to achieve high-throughput epitope mapping of all 13 hexon proteins in B and C species HAdVs. This study has demonstrated that all of the epitopes from the 13 hexon proteins are located in the proteins' tower regions; however, the exact number, location, and size of the epitopes differ among the HAdV serotypes.

分类号：

  • 相关文献
作者其他论文 更多>>

微信小程序