Characterization of Interferon-alpha 1 of Yak

文献类型： 外文期刊

第一作者： Chen, GuoHua

作者： Chen, GuoHua;Jia, HuaiJie;Zhen, YaDong;Zeng, Shuang;Fang, YongXiang;He, XiaoBing;Jing, ZhiZhong;Cai, XuePeng

作者机构：

关键词： Yak (Bos grunniens);interferon-alpha 1;cloning;antiviral activity assay;Pichia pastoris

期刊名称：JOURNAL OF ANIMAL AND VETERINARY ADVANCES （ 影响因子：0.365； 五年影响因子：0.359 ）

ISSN： 1680-5593

年卷期： 2012 年 11 卷 21 期

页码：

收录情况： SCI

摘要： The yak (Bos grunniens) is an important grazing livestock in Western of China. In this study, the full length of yIFN-a1 gene was cloned then the recombinant yIFN-a1 was expressed in. yeast expression system. In vitro bioactivity of IFN-alpha 1 was determined by inhabition infection BHK cell against Vesicular Stomatitis Virus (VSV). The results showed that cDNA contains an Open Reading Frame (ORF) of 570 base pairs encoding a protein. of 189 amino acid (aa) precursor with a signal peptide of 23 aa. The homology of the yIFN-a1 was up to 65.3 similar to 94.4% with other species at the amino acid levels. The recombinant yIFN-a1 was recognized in Western blot by mAb against human IFN-alpha 1. The activity unit of the purified recombinant yIFN-a1 protein was 1.16x10(5) IU mg(-1) by against Vesicular Stomatitis Virus (VSV) antiviral assay in vitro. This made a basis for the development of anti-virus medicine of yak in the future.

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