Rapid detection of Betacoronavirus 1 from clinical fecal specimens by a novel reverse transcription loop-mediated isothermal amplification assay

文献类型： 外文期刊

第一作者： Qiao, Jun

作者： Qiao, Jun;Meng, Qingling;Chen, Chuangfu;Zhang, Zaichao;Tian, Zhenzhong;Cai, Xuepeng

作者机构：

关键词： Betacoronavirus 1;nucleocapsid gene;rapid detection;reverse transcription loop-mediated isothermal amplification

期刊名称：JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION （ 影响因子：1.279； 五年影响因子：1.522 ）

ISSN： 1040-6387

年卷期： 2012 年 24 卷 1 期

页码：

收录情况： SCI

摘要： Betacoronavirus 1 (BCoV-1) is an important pathogen causing diarrhea in calves. In the current study, a novel reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for rapid detection of BCoV-1 was successfully developed. The primers were designed to target the highly conserved fragment of BCoV-1 nucleocapsid gene. The assay displayed high specificity detecting only BCoV-1 with no cross reaction with other viruses. When 418 clinical samples from 6 different geographical areas of Xinjiang province were tested by the RT-LAMP method, the results indicated that this test is a simple, rapid, accurate, and sensitive method for the detection of BCoV-1.

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