Scarabaeid Larvae- and Herbicide-Resistant Transgenic Perennial Ryegrass (Lolium perenne L.) Obtained by Agrobacterium tumefaciens-Mediated Transformation of cry8Ca2, cry8Ga and bar Genes

文献类型： 外文期刊

第一作者： Wu Jin-xia

作者： Wu Jin-xia;Zhang Zhi-guo;Zhang Qian;Lang Zhi-hong;Sun Xue-hui

作者机构：

关键词： Agrobacterium tumefaciens-mediated transformation;transgenic perennial ryegrass;scarab larvae resistance;herbicide resistance

期刊名称：JOURNAL OF INTEGRATIVE AGRICULTURE （ 影响因子：2.848； 五年影响因子：2.979 ）

ISSN： 2095-3119

年卷期： 2012 年 11 卷 1 期

页码：

收录情况： SCI

摘要： Insect pest and weeds are two major problems for forage and turf grasses. In this study, scarab larvae- and herbicide-resistant transgenic perennial ryegrass (Lolium perenne L.) was obtained by transforming it with cry and bar genes simultaneously via the Agrobacterium-mediated method. To optimize the callus induction and plant regeneration conditions, various concentrations of 2,4-dichlorophenoxyacetic acid and 6-benzylaminopurine were assayed. The transformation efficiencies of different Agrobacterium suspension media, used during Agrobacterium-mediated transformation, were compared. Then, plasmids of pCAMBIA3301 containing cry gene (cry8Ca2 or cry8Ga) and bar gene, driven by ubiquitin promoter, were transformed into perennial ryegrass. The transformants were generated and confirmed by both Southern hybridization analysis and Western hybridization analysis. Further, the resistance of transgenic perennial ryegrass plants to scarab larvae and herbicide were analyzed. After 30 d of co-cultivation with scarab larvae, the damage to the root system of transgenic plants was less than that of non-transgenic control plants. Additionally, the leaves of transgenic plants were resistant to Basta (R), while leaves of the wild plants wilted after Basta (R) spraying. These results show that cry gene and bar gene were successfully transferred into perennial ryegrass by the Agrobactgerium-mediated method, and convey resistance to scarab larvae and herbicide in transgenic perennial ryegrass plants.

分类号：

  • 相关文献

[1]Establishment of an efficient transformation system for Pleurotus ostreatus. Lei, Min,Wu, Xiangli,Zhang, Jinxia,Huang, Chenyang,Lei, Min,Wang, Hexiang. 2017

[2]Development of a simple and efficient transformation system for the basidiomycetous medicinal fungus Ganoderma lucidum. Shi, Liang,Fang, Xing,Li, Mengjiao,Mu, Dashuai,Ren, Ang,Zhao, Mingwen,Tan, Qi. 2012

[3]Optimization of Agrobacterium tumefaciens-Mediated Immature Embryo Transformation System and Transformation of Glyphosate-Resistant Gene 2mG2-EPSPS in Maize (Zea mays L.). Liu Yong-sheng,Yu Gui-rong,Du Wen-ping,Song Jun,Xu Li-yuan,Liu Yan,Lin Min,Xiao Fang-ming. 2013

[4]Expression and inheritance of Bt cry1Ia8 gene in transgenic cabbage to control Plutella xylostella. Yi, Dengxia,Fang, Zhiyuan,Yang, Limei. 2017

[5]CRISPR/Cas9-mediated targeted mutagenesis of GmFT2a delays flowering time in soya bean. Cai, Yupeng,Chen, Li,Liu, Xiujie,Guo, Chen,Hou, Wensheng,Cai, Yupeng,Chen, Li,Liu, Xiujie,Guo, Chen,Sun, Shi,Wu, Cunxiang,Jiang, Bingjun,Han, Tianfu,Hou, Wensheng. 2018

[6]Development of Alfalfa (Medicago sativa L.) Regeneration System and Agrobacterium-Mediated Genetic Transformation. Zhang He,Huang Qi-man,Su Jin. 2010

[7]A simple and efficient transformation system for the edible mushroom Pleurotus eryngii. Wang, Ying,Wang, Rong,Mao, Wen Jun,Gong, Ming,Gao, Ying Nv,Tang, Li Hua,Li, Yan,Zhou, Chen Li,Bao, Da Peng,Yang, Rui Fang.

[8]Establishment of Agrobacterium tumefaciens-mediated transformation system for Phomopsis asparagi, the pathogen of asparagus stem blight. Yang, Ying-Qing,Lan, Bo,Jian, Yan-Li,Hu, Shui-Xiu,Chang, Dong-Dong,Zhang, Shun-Liang,Li, Xiang-Min,Jian, Yan-Li,Hu, Shui-Xiu,Chang, Dong-Dong. 2015

[9]Bidirectional promoter trapping T-DNA for insertional mutagenesis in Verticillium dahliae. Deng, Sheng,Wang, Cai-yue,Zhang, Xin,Lin, Ling.

[10]Resistance to ACCase-inhibiting herbicides in an Asia minor bluegrass (Polypogon fugax) population in China. Chen, Jie,Zhou, Fengyan,Zhou, Xiaogang. 2014

[11]Inheritance and molecular characterization of resistance to AHAS-inhibiting herbicides in rapeseed. Hu Mao-long,Pu Hui-ming,Gao Jian-qin,Long Wei-hua,Chen Feng,Zhou Xiao-ying,Zhang Wei,Peng Qi,Chen Song,Zhang Jie-fu. 2017

[12]Resistance evaluation for herbicide resistance-endowing acetolactate synthase (ALS) gene mutations using Raphanus raphanistrum populations homozygous for specific ALS mutations. Yu, Q.,Han, H.,Purba, E.,Walsh, M. J.,Powles, S. B.,Li, M.,Purba, E.. 2012

[13]Transgenic rice expressing a novel phytase-lactoferricin fusion gene to improve phosphorus availability and antibacterial activity. Deng Li-hua,Weng Lu-shui,Deng Xiang-yang,Xiao Guo-ying,Wang Zuo-ping,Fu Xi-qin,Xin Ye-yun. 2017

[14]Efficient Sugarcane Transformation via bar Gene Selection. Wang, W. Z.,Yang, B. P.,Feng, C. L.,Wang, J. G.,Xiong, G. R.,Zhao, T. T.,Zhang, S. Z.. 2017

[15]Acetolactate Synthase Gene Pro line (197) Mutations Confer Tribenuron-Methyl Resistance in Flixweed (Descurainia sophia) Populations from China. Zhang, Chao Xian,Wei, Shou Hui,Li, Xiang Ju,Cui, Hai Lan,Zhang, Chao Xian,Wei, Shou Hui,Li, Xiang Ju,Zhang, Hong Jun,Zhang, Yan Qiu,Wang, Gui Qi.

[16]Molecular basis of resistance to imazethapyr in redroot pigweed (Amaranthus retroflexus L.) populations from China. Chen, Jinyi,Huang, Zhaofeng,Zhang, Chaoxian,Huang, Hongjuan,Wei, Shouhui,Chen, Jingchao,Wang, Xu.

[17]iTRAQ-based quantitative proteomic analysis reveals proteomic changes in three fenoxaprop-P-ethyl-resistant Beckmannia syzigachne biotypes with differing ACCase mutations. Zhang, Jian,Wang, Junzhi,Dong, Liyao,Pan, Lang,Zhang, Jian,Dong, Liyao,Yu, Qin,Bai, Lianyang.

[18]Transcriptomics analysis of the flowering regulatory genes involved in the herbicide resistance of Asia minor bluegrass (Polypogon fugax). Zhou, Fengyan,Zhang, Yong,Wang, Mei,Gao, Tongchun,Tang, Wei. 2017

[19]Overexpression of D-amino acid oxidase from Bradyrhizobium japonicum, enhances resistance to glyphosate in Arabidopsis thaliana. Han, Hongjuan,Zhu, Bo,Fu, Xiaoyan,You, Shuanghong,Wang, Bo,Li, Zhenjun,Zhao, Wei,Peng, Rihe,Yao, Quanhong,You, Shuanghong,Yao, Quanhong.

[20]Acetolactate synthase proline (197) mutations confer tribenuron-methyl resistance in Capsella bursa-pastoris populations from China. Li, Xiangju,Wei, Shouhui,Cao, Hongyu,Wang, Guiqi,Wang, Jianping. 2012

作者其他论文 更多>>

微信小程序