Immune Effect of Newcastle Disease Virus DNA Vaccine with C3d as a Molecular Adjuvant

文献类型: 外文期刊

第一作者: Zhao, Kai

作者: Zhao, Kai;Duan, Xutong;Hao, Lianwei;Wang, Xiaohua;Zhao, Kai;Duan, Xutong;Wang, Yunfeng

作者机构:

关键词: Newcastle disease virus;DNA vaccine;F gene;C3d;molecular adjuvant

期刊名称:JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY ( 影响因子:2.351; 五年影响因子:2.65 )

ISSN: 1017-7825

年卷期: 2017 年 27 卷 11 期

页码:

收录情况: SCI

摘要: Newcastle disease is a serious infectious disease in the poultry industry. The commercial vaccines can only offer limited protection and some of them are expensive and need adjuvants. At present, DNA vaccines are widely used. However, the immune responses induced by DNA vaccines are too slow and low. Here, we constructed the transfer vectors with a different number of C3d as molecular adjuvants (n = 1, 2, 4, or 6), and the vectors were cloned into the optimal eukaryotic expression plasmid (pVAXI-optiF) that expressed the F gene of Newcastle disease virus (NDV), and named pVAXI-F(o)-C3d1, pVAXI -F(o)-C3d2, pVAXI-F(o)-C3d4, and pVAXI-F(o)-C3d6, respectively. Cell transfection test indicated that pVAXI-F(o)-C3d6 showed the highest expression. In vivo immunization showed that the chickens immunized with pVAXI-F(o)-C3d6 intramuscularly induced better immune responses than the chickens immunized with the other plasmids. The protective efficacy of pVAXI-F(o)-C3d6 was 80% after challenge with the highly virulent NDV strain F48E9. The results in this study showed that C3d6 could be used as a molecular adjuvant to quickly induce an effective immune response to control NDV.

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