DNA demethylation activates genes in seed maternal integument development in rice (Oryza sativa L.)
文献类型: 外文期刊
第一作者: Wang, Yifeng
作者: Wang, Yifeng;Tong, Xiaohong;Hou, Yuxuan;Zhang, Jian;Lin, Haiyan;Chang, Yuxiao
作者机构:
关键词: Rice (Oryza sativa L.);DNA methylation;Seed integument development;Demethylation
期刊名称:PLANT PHYSIOLOGY AND BIOCHEMISTRY ( 影响因子:4.27; 五年影响因子:4.816 )
ISSN: 0981-9428
年卷期: 2017 年 120 卷
页码:
收录情况: SCI
摘要: DNA methylation is an important epigenetic modification that regulates various plant developmental processes. Rice seed integument determines the seed size. However, the role of DNA methylation in its development remains largely unknown. Here, we report the first dynamic DNA methylomic profiling of rice maternal integument before and after pollination by using a whole-genome bisulfite deep sequencing approach. Analysis of DNA methylation patterns identified 4238 differentially methylated regions underpin 4112 differentially methylated genes, including GW2, DEP1, RGB1 and numerous other regulators participated in maternal integument development. Bisulfite sanger sequencing and qRT-PCR of six differentially methylated genes revealed extensive occurrence of DNA hypomethylation triggered by double fertilization at IAP compared with IBP, suggesting that DNA demethylation might be a key mechanism to activate numerous maternal controlling genes. These results presented here not only greatly expanded the rice methylome dataset, but also shed novel insight into the regulatory roles of DNA methylation in rice seed maternal integument development. (C) 2017 Elsevier Masson SAS. All rights reserved.
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