Heteroallelic diploid pollen led to self-compatibility in tetraploid cultivar 'Sha 01' (Pyrus sinkiangensis Yu)

文献类型： 外文期刊

第一作者： Qi, Yong-Jie

作者： Qi, Yong-Jie;Wu, Hua-Qing;Wu, Jun;Tao, Shu-Tian;Zhang, Shao-Ling;Cao, Yu-Fen

作者机构：

关键词： 'Sha 01' (Pyrus sinkiangensis Yu);Tetraploid;Self-compatibility;S-RNase gene;Segregation

期刊名称：TREE GENETICS & GENOMES （ 影响因子：2.297； 五年影响因子：2.547 ）

ISSN： 1614-2942

年卷期： 2011 年 7 卷 4 期

页码：

收录情况： SCI

摘要： Pear cultivar 'Sha 01' is a sport from 'Kuerlexiangli'. Field pollination data revealed that 'Sha 01' displayed self-compatibility (SC), whereas 'Kuerlexiangli' showed self-incompatibility upon self-pollination. Reciprocal pollinations between the two varieties showed that 76% of 'Kuerlexiangli' flowers pollinated with 'Sha 01' pollen set fruit, but only 7% of 'Sha 01' flowers set fruit when pollinated with 'Kuerlexiangli' pollen. The pollen performance was monitored with fluorescence microscopy, and it was observed that 'Sha 01' accepted self-pollen but rejected 'Kuerlexiangli' pollen, whereas 'Kuerlexiangli' rejected self-pollen but accepted 'Sha 01' pollen. Taken together, 'Sha 01' showed SC as a pollen-part mutant. Molecular S-genotyping of 'Sha 01', its selfed progeny, and wild-type 'Kuerlexiangli' showed that all contained S (22)-RNase and S (28)-RNase alleles, but showed no nucleotide sequence difference or changes in transcription. After flow cytometry and chromosome number analyses, 'Sha 01' was found to be a tetraploid (2n = 68) and 'Kuerlexiangli' a diploid (2n = 34). Thus, the S-genotype for 'Sha 01' was S (22) S (22) S (28) S (28) and for 'Kuerlexiangli' was S (22) S (28) . Ploidy level of 'Sha 01' selfed progeny were also determined from leaves using flow cytometry; all seedlings were tetraploids. The S-genotyping for the progeny was identified by S-RNase gene relative optical density for both alleles. Closer inspection of 52 progeny showed that they could be classified into three types S (22) S (22) S (28) S (28) : S (22) S (22) S (22) S (28) : S (22) S (28) S (28) S (28) with the distribution 28:10:14 a parts per thousand aEuro parts per thousand 4:1:1; no individual homozygous for either allele was found. Therefore, it could be concluded that only heteroallelic diploid pollen S (22) S (28) could achieve 'Sha 01' fertilization through a so-called competitive interaction.

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