Transformation of Two VP1 Genes of O- and Asia 1-Type Foot-and-Mouth Disease Virus into Maize

文献类型： 外文期刊

第一作者： Zhang Su-zhi

作者： Zhang Su-zhi;Zhang Gui-ling;Rong Ting-zhao;Zhang Su-zhi;Pan Li;Zhou Peng;Zhang Yong-guang

作者机构：

关键词： FMDV;P12A3C gene;maize;transformation;edible vaccine

期刊名称：AGRICULTURAL SCIENCES IN CHINA （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2011 年 10 卷 5 期

页码：

收录情况： SCI

摘要： The expression of antigens in transgenic plants has increasingly been used as an alternative to the classical methodologies for the development of experimental vaccines. This paper reports here the development of a novel oral immunization system for foot-and-mouth disease (FMD) in transgenic maize with two serotypes of the structural protein VP1 of the foot-and-mouth disease virus (FMDV) viz., O- and Asia 1-type, respectively. The transgenic plantlets were identified and investigated by polymerase chain reaction (PCR), Southern blot, and real-time PCR. Moreover, it was found that the VP1 genes in transgenic plants could be transmitted stably to the next generation through PCR detection. To our knowledge, this is the first report in an attempt to induce a protective systemic antibody response in animals by feeding the transgenic plants in which two scrotypes antigen protein of FMDV expressed together. Results of the experiment provide the possibility of using plant-based vaccines as feedstuff or feedstuff additives.

分类号：

  • 相关文献

[1]Research Progress on Transformation Maize Mediated by Agrobacterithm Tumefaciens. Li, Xiuping,Li, Xiuping,Jiang, Lijing,Liu, Na. 2011

[2]Comparison of immune responses against foot-and-mouth disease virus induced by fusion proteins using the swine IgG heavy chain constant region or beta-galactosidase as a carrier of immunogenic epitopes. Li, GJ,Chen, WZ,Yan, WY,Zhao, K,Liu, MQ,Zhang, J,Fei, L,Xu, QX,Sheng, ZT,Lu, YG,Zheng, ZX.

[3]Protective efficacy against Chlamydophila psittaci by oral immunization based on transgenic rice expressing MOMP in mice. Zhang, Xiu-Xiang,Zhang, Xiu-Xiang,Yu, Hui,Wang, Xiao-Hu,Luo, Sheng-Jun,Li, Xiu-Zhen,Li, Hao-Xin,Yuan, Zi-Guo,Zhu, Yan-Ping.

[4]Histological and Ultrastructural Observation Reveals Significant Cellular Differences between Agrobacterium Transformed Embryogenic and Non-embryogenic Calli of Cotton. Shang, Hai-Hong,Liu, Chuan-Liang,Zhang, Chao-Jun,Li, Feng-Lian,Hong, Wei-Dong,Li, Fu-Guang.

[5]Cross-inhibition to heterologous foot-and-mouth disease virus infection induced by RNA interference targeting the conserved regions of viral genome. Liu, MQ,Chen, WZ,Ni, Z,Yan, WY,Fei, L,Jiao, Y,Zhang, J,Du, QY,Wei, X,Chen, JL,Liu, YM,Zheng, ZX.

[6]Expression of foot-and-mouth disease virus epitopes in tobacco by a tobacco mosaic virus-based vector. Wu, LG,Jiang, LB,Zhou, ZA,Fan, JH,Zhang, QQ,Zhu, HH,Han, Q,Xu, ZK.

[7]Long-term humoral immunity induced by CVC1302-adjuvanted serotype O foot-and-mouth disease inactivated vaccine correlates with promoted T follicular helper cells and thus germinal center responses in mice. Zheng, Qisheng,Hou, Jibo.

[8]Immunogenicity of T7 bacteriophage nanoparticles displaying G-H loop of foot-and-mouth disease virus (FMDV). Xu, Hai,Bao, Xi,Lu, Yu,Liu, Yamei,Deng, Bihua,Wang, Yiwei,Xu, Yue,Hou, Jibo,Xu, Hai,Lu, Yu,Hou, Jibo.

[9]Bovine fetal epithelium cells expressing shRNA targeting viral VP1 gene resisted against foot-and-mouth disease virus. Wang, Hongmei,Liu, Xiao,Wu, Jianming,Wu, Gang,Yang, Huiting,Xie, Weixin,He, Hongbin,Yu, Li,He, Chenqiang,Xia, Xianzhu.

[10]Foot-and-mouth disease vaccines: progress and problems. Cao, Yimei,Lu, Zengjun,Liu, Zaixin.

[11]The VP1 S154D mutation of type Asia1 foot-and-mouth disease virus enhances viral replication and pathogenicity. Lian, Kaiqi,Yang, Fan,Zhu, Zixiang,Cao, Weijun,Jin, Ye,Liu, Huanan,Li, Dan,Zhang, Keshan,Guo, Jianhong,Liu, Xiangtao,Zheng, Haixue.

[12]Plus- and minus-stranded foot-and-mouth disease virus RNA quantified simultaneously using a novel real-time RT-PCR. Gu, Chaojiang,Zheng, Congyi,Shi, Lili,Zhang, Qian,Li, Yong,Lu, Bin,Xiong, Yi,Qu, Sanpu,Shao, Junjun,Chang, Huiyun. 2007

[13]Resiquimod and polyinosinic-polycytidylic acid formulation with aluminum hydroxide as an adjuvant for foot-and-mouth disease vaccine. Zhou, Chun-Xue,Li, Dong,Chen, Ying-Li,Lu, Zeng-Jun,Sun, Pu,Cao, Yi-Mei,Bao, Hui-Fang,Fu, Yuan-Fang,Li, Ping-Hua,Bai, Xing-Wen,Xie, Bao-Xia,Liu, Zai-Xin. 2014

[14]Nucleotide mismatches of foot-and-mouth disease virus during replication. Ma, Y. B.,Hao, C. X.,Chang, H. Y.. 2013

[15]Foot-and-mouth disease virus carrier status in Bos grunniens yaks. Chang, Huiyun,Ma, Yanbin,Lin, Tong,Cong, Guozheng,Du, Junzheng,Ma, Jinling. 2013

[16]The pH stability of foot-and-mouth disease virus. Yuan, Hong,Li, Pinghua,Ma, Xueqing,Lu, Zengjun,Sun, Pu,Bai, Xingwen,Zhang, Jing,Bao, Huifang,Cao, Yimei,Li, Dong,Fu, Yuanfang,Chen, Yingli,Zhang, Jie,Liu, Zaixin,Bai, Qifeng. 2017

[17]Geometrical Stuidy on FMDV Genome Based on Z-Curve. Tang, Hua,Liu, Xin-Sheng,Fang, Yu-Zhen,Pan, Li,Zhang, Zong-Wang,Zhou, Peng,Lv, Jian-Liang,Jiang, Shou-Tian,Wang, Yong-Lu,Zhang, Yong-Guang. 2012

[18]Induction of mucosal immune responses and protection of cattle against direct-contact challenge by intranasal delivery with foot-and-mouth disease virus antigen mediated by nanoparticles. Pan, Li,Zhang, Zhongwang,Lv, Jianliang,Zhou, Peng,Hu, Wenfa,Fang, Yuzhen,Chen, Haotai,Liu, Xinsheng,Shao, Junjun,Zhao, Furong,Ding, Yaozhong,Lin, Tong,Chang, Huiyun,Zhang, Jie,Zhang, Yongguang,Wang, Yonglu,Zhang, Zhongwang,Lv, Jianliang,Zhou, Peng,Hu, Wenfa,Fang, Yuzhen,Chen, Haotai,Liu, Xinsheng,Shao, Junjun,Zhao, Furong,Ding, Yaozhong,Lin, Tong,Chang, Huiyun,Zhang, Jie,Zhang, Yongguang,Wang, Yonglu. 2014

[19]Immune responses of recombinant adenovirus co-expressing VP1 of foot-and-mouth disease virus and porcine interferon alpha in mice and guinea pigs. Du, Yijun,Dai, Jianjun,Li, Yufeng,Duan, Shuyi,Jiang, Ping,Li, Congzhi,Qi, Jing. 2008

[20]Poly(I:C) combined with multi-epitope protein vaccine completely protects against virulent foot-and-mouth disease virus challenge in pigs. Cao, Yimei,Lu, Zengjun,Li, Yanli,Sun, Pu,Li, Dong,Li, Pinghua,Bai, Xingwen,Fu, Yuanfang,Bao, Huifang,Zhou, Chunxue,Xie, Baoxia,Chen, Yingli,Liu, Zaixin. 2013

作者其他论文 更多>>

微信小程序