Cloning, expression and preliminary crystallographic analysis of the equine infectious anaemia virus (EIAV) gp45 ectodomain

文献类型： 外文期刊

第一作者： Sun, Pei-Long

作者： Sun, Pei-Long;Liu, Xin-Qi;Sun, Pei-Long;Lv, Shu-Xia;Sun, Pei-Long;Zhou, Jian-Hua

作者机构：

关键词： equine infectious anaemia virus;gp45 ectodomain;lentiviruses

期刊名称：ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS （ 影响因子：0.524； 五年影响因子：0.451 ）

ISSN： 1744-3091

年卷期： 2011 年 67 卷

页码：

收录情况： SCI

摘要： Like human immunodeficiency virus (HIV), equine infectious anaemia virus (EIAV) belongs to the lentivirus genus. The first successful lentiviral vaccine was developed for EIAV. Thus, EIAV may serve as a valuable model for HIV vaccine research. EIAV glycoprotein 45 (gp45) plays a similar role to gp41 in HIV by mediating virus-host membrane fusion. The gp45 ectodomain was constructed according to the structure of HIV gp41, with removal of the disulfide-bond loop region. The protein was expressed in Escherichia coli and crystallized following purification. However, most of the crystals grew as aggregates and could not be used for data collection. By extensively screening hundreds of crystals, a 2.7 A resolution data set was collected from a single crystal. The crystal belonged to space group P6(3), with unit-cell parameters a = b = 46.84, c = 101.61 A, alpha = beta = 90, gamma = 120 degrees. Molecular replacement was performed using the coordinates of various lengths of HIV gp41 as search models. A long bent helix was identified and a well defined electron-density map around the long helix was obtained. This primary model provided the starting point for further refinement.

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