Molecular Mechanism of HINTW (HINT Gene in W-Chromosome)-Mediated UBE2I (Ubiquitin Conjugating Enzyme E2 I) Interaction in Female Differentiation of Chicken Embryos
文献类型： 外文期刊
第一作者： Sun, Changhua
作者： Sun, Changhua;Sun, Changhua;Ali, Malik Ahsan;Sun, Hongyan;Niu, Yingjie;Zuo, Qisheng;Li, Bichun;Jin, Kai;Sun, Changhua;Ali, Malik Ahsan;Sun, Hongyan;Niu, Yingjie;Zuo, Qisheng;Li, Bichun;Jin, Kai;Sun, Changhua;Ali, Malik Ahsan;Jin, Kai;Song, Jiuzhou;Li, Bichun;Ali, Malik Ahsan;Zuo, Qisheng;Jin, Kai;Han, Wei;Li, Bichun;Li, Bichun
作者机构：
关键词： HINTW; UBE2I; female differentiation; chicken embryo; pull-down assay
期刊名称：GENES （ 影响因子：2.8； 五年影响因子：3.2 ）
ISSN：
年卷期： 2025 年 16 卷 4 期
页码：
收录情况： SCI
摘要： Background/Objective: It has been established that HINTW plays a pivotal role in the female differentiation of chickens; nevertheless, the underlying molecular mechanism remains to be fully elucidated. Method: To investigate the role of HINTW in avian sex determination, a prokaryotic expression vector containing its key structural domain was constructed, and its in vitro expression was achieved. Pull-down assays were performed to capture interacting proteins from male and female gonadal tissues, followed by a silver staining analysis to compare interaction profiles between ovaries and testes. Mass spectrometry was utilized to identify differentially bound proteins. Additionally, functional characterization and co-immunoprecipitation (Co-IP) assays were conducted to validate the interaction between HINTW and its candidate binding partner. Result: A total of 1590 differentially bound proteins were identified between ovarian and testicular tissues. Functional analysis and Co-IP assays confirmed a specific interaction between HINTW and UBE2I in the ovary, suggesting that HINTW may facilitate female differentiation in chicken embryos through its interaction with UBE2I. Conclusions: This study provides novel insights into the molecular mechanisms underlying HINTW-mediated female differentiation in chickens and contributes to a deeper understanding of avian sex determination.
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