Crystallization and preliminary X-ray analysis of a novel esterase Rv0045c from Mycobacterium tuberculosis

文献类型： 外文期刊

第一作者： Xu, Lipeng

作者： Xu, Lipeng;Liu, Siguo;Xu, Lipeng;Guo, Jiubiao;Zheng, Xiangdong;Pang, Hai;Wen, Tingyi;Sun, Fei

作者机构：

关键词： RV0045c;Mycobacterium tuberculosis;esterases;lipid metabolism

期刊名称：ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS （ 影响因子：0.524； 五年影响因子：0.451 ）

ISSN： 1744-3091

年卷期： 2010 年 66 卷

页码：

收录情况： SCI

摘要： The Rv0045c protein is predicted to be an esterase that is involved in lipid metabolism in Mycobacterium tuberculosis. The protein was overproduced in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. The Rv0045c protein crystals diffracted to a resolution of 2.7 A using a synchrotron-radiation source and belonged to space group P3(1) or P3(2), with unit-cell parameters a = b = 73.465, c = 48.064 A, alpha = beta = 90, gamma = 120 degrees. Purified SeMet-labelled Rv0045c protein was also crystallized and formed crystals that diffracted to a resolution of 3.0 A using an in-house X-ray radiation source.

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