Bio-inorganic Synthesis of ZnO Powders Using Recombinant His-tagged ZnO Binding Peptide as a Promoter

文献类型： 外文期刊

第一作者： Song, Lei

作者： Song, Lei;Chen, Jinchun;Liu, Yingying;Zhang, Zhifang;Wang, Xi

作者机构：

关键词： Recombinant technology;Expression and purification;Specific binding protein;Biosorption;Precursor precipitates;Bio-inorganic synthesis

期刊名称：PROTEIN JOURNAL （ 影响因子：2.371； 五年影响因子：1.929 ）

ISSN： 1572-3887

年卷期： 2010 年 29 卷 7 期

页码：

收录情况： SCI

摘要： Inorganic-binding peptides termed as genetically engineered polypeptides for inorganics (GEPIs), are small peptide sequences selected via combinatorial biology-based protocols of phage or cell surface display technologies. Recent advances in nanotechnology and molecular biology allow the engineering of these peptides with specific affinity to inorganics, often used as molecular linkers or assemblers, to facilitate materials synthesis, which provides a new insight into the material science and engineering field. As a case study on this biomimetic application, here we report a novel biosynthetic ZnO binding protein and its application in promoting bio-inorganic materials synthesis. In brief, the gene encoding a ZnO binding peptide(ZBP) was genetically fused with His(6)-tag and GST-tag using E.coli expression vector pET-28a (+) and pGEX-4T-3. The recombinant protein GST-His-ZBP was expressed, purified with Ni-NTA system, identified by SDS-PAGE electrophoresis and Western blot analysis and confirmed by liquid chromatography-mass spectrometry/mass spectrometry (LC-MS/MS) analysis. Affinity adsorption test demonstrated that the fusion protein had a specific avidity for ZnO nanoparticles (NPs). Results from the bio-inorganic synthesis experiment indicated that the new protein played a promoting part in grain refinement and accelerated precipitation during the formation of the ultra-fine precursor powders in the Zn(OH)(2) sol. X-ray diffraction (XRD) analysis on the final products after calcining the precursor powders showed that hexagonal wurtzite ZnO crystals were obtained. Our work suggested a novel approach to the application about the organic-inorganic interactions.

分类号：

  • 相关文献

[1]Cloning, expression, and purification of an antiviral protein from Pseudomonas fluorescens CZ and its antagonistic activity against tobacco mosaic virus. Shen, Lili,Jiao, Yubing,Wu, Yuanhua,Shen, Lili,Li, Fangfang,Sun, Hangjun,Wang, Fenglong.

[2]The Fabrication of Bio-ceramsite for the Removal of Heavy Metals and Its Toxicity to Bacteria. Shi Yan,Qi Xuebin,Gao Qing,Shi Yan,Sun Ken,Sun Ken. 2015

[3]Bioremediation of wastewaters with decabromodiphenyl ether by anaerobic granular sludge. Ni, Shou-Qing,Wang, Zhibin,Lv, Lu,Liang, Xueyou,Ren, Longfei,Wang, Zhibin,Zhou, Qingxin.

[4]Ammonium adsorption from aqueous solutions by strawberry leaf powder: Equilibrium, kinetics and effects of coexisting ions. Liu, Haiwei,Dong, Yuanhua,Wang, Haiyun,Liu, Yun,Liu, Haiwei,Liu, Haiwei.

[5]Cross-linked chitosan polymers as generic adsorbents for simultaneous adsorption of multiple mycotoxins. Zhao, Zhiyong,Wu, Aibo,Zhao, Zhiyong,Yang, Lingchen,Song, Suquan,Hou, Jiafa,Zhao, Zhiyong,Liu, Na,Wang, Jianhua,Nie, Dongxia,Yang, Xianli.

[6]Recovery of Palladium(II) from nitric acid medium using a natural resin prepared from persimmon dropped fruits residues. Yi, Qingping,Fan, Ruiyi,Zhang, Qinglin,Luo, Zhengrong,Yi, Qingping,Xie, Feng,Zhang, Qinglin,Luo, Zhengrong.

[7]Lead biosorption of probiotic bacteria: effects of the intestinal content from laying hens. Xing, Sicheng,Liao, Xindi,Wang, Jie,Liang, Juan Boo,Jahromi, Mohammad Faseleh,Shokryazdan, Parisa,Jahromi, Mohammad Faseleh,Shokryazdan, Parisa,Zhu, Cui,Laudadio, Vito,Tufarelli, Vincenzo.

作者其他论文 更多>>

微信小程序