Expression of IL-13R alpha 2 in liver cancer cells and its effect on targeted therapy of liver cancer

文献类型: 外文期刊

第一作者: Hou, Lingling

作者: Hou, Lingling;Du, Juan;Liu, Yanfeng;Sun, Weimin;Zheng, Yanpeng;Zhang, Lishu;Hu, Honggang;Dai, Xinxian;Hong, Tao;Wang, Jianwei;Guan, Weijun;Ma, Yuehui

作者机构:

关键词: Liver cancer;IL-13R alpha 2;Targeted therapy;siRNA

期刊名称:JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY ( 影响因子:4.553; 五年影响因子:4.201 )

ISSN: 0171-5216

年卷期: 2010 年 136 卷 6 期

页码:

收录情况: SCI

摘要: Liver cancer is the third leading cause of cancer-related deaths globally. The number of liver cancers diagnosed in the world is increasing at an alarming rate. It is of great significance to find the new targets of the tumor cells and specific medicine. This research investigated the expression of interleukin-13 receptor alpha 2 (IL-13R alpha 2) in different liver cancer cell lines and liver cancer tissues, and assessed the cytotoxin DT389-hIL13-13E13K (IL-13 and diphtheria toxin fusion protein) targeted killing effect on liver cancer cells. Based on study above, we further analyzed the function of IL-13R alpha 2 on the targeted liver cancer therapy. The results will provide a novel strategy and an alternative way for liver cancer therapy. The expression of IL-13R alpha 2 in different liver cancer cell lines and tissues were analyzed by RT-PCR and immunohistochemistry. Cytotoxicity assay of DT389-hIL13-13E13K was performed in eight different concentrations in liver cancer cell lines in vitro. At the same time, siRNA-mediated knockdown was introduced to assess the role of IL-13R alpha 2 in liver cancer therapy. Two out of four tested liver cancer cell lines and 27 out of 33 (81.82%) liver tissues expressed the IL-13R alpha 2. The fusion protein DT(389)-hIL13-13E13K showed a moderate cytotoxicity to the cancer cell line BEL-7402 in vitro, which 50% inhibition (IC(50)) concentration occurred at 1.4 x 10(-5) M. Besides, the sensitivity to fusion protein DT(389)-hIL13-13E13K was decreased in siRNA-transfected liver cells compared with control ones. These results suggest that IL-13R alpha 2 chain is a specific target for IL-13-directed fusion protein. We reported the expression of IL-13R alpha 2 in liver cancer cell lines and tissues as well as investigated the cytotoxin (DT389-hIL13-13E13K) targeted killing efficiency of liver cancer cells and potential role of IL-13R alpha 2 in the cancer treatment.

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