Gene Cloning and Tissue-Specific Expression of G Protein beta Subunit in Microplitis mediator (Hymenoptera: Braconidae)

文献类型： 外文期刊

第一作者： ZHANG Shuai

作者： ZHANG Shuai;ZHANG Yong-jun;CUI Jin-jie;GAO Xi-wu;GUO Yu-yuan

作者机构：

关键词： Microplitis mediator;G protein beta subunit;quantitative real-time RT-PCR;expression pattern

期刊名称：Agricultural Sciences in China （ 影响因子：0.82； 五年影响因子：0.997 ）

ISSN： 1671-2927

年卷期： 2010 年 9 卷 4 期

页码：

收录情况： SCI

摘要： A gene encoding a novel G protein beta subunit of beta 1 subclass, G beta Mmed was isolated from Microfilms mediator (Hymenoptera. Braconidae) The full-length sequence of G beta Mmed is 1 119 bp, the cDNA contains a 1 023 bp open reading frame that encodes a protein with 340 ammo acids, and the predicted molecular weight of G beta Mmed is 37 23 kDa and isoelectric point is 5 86. By the quantitative real-time RT-PCR method, the tissue-specific expression and quantitative changes in the developmental expression profile of G beta Mmed were detected It was found that G beta Mmed was abundantly expressed in M. mediator antennae, head (without antennae), thorax, abdomen, legs and the wings. and especially at high levels in abdomen. In antennae, expression varied through 1st day before emergence to 5-d-old adults, and had equal expression levels detected in females and males in total In head, G beta Mmed expresses while initially high in females, and have another peaked in stage 4 and 1st day. in males showed a peak of G beta Mmed expression prior to emergence and relatively low levels after emergence In female abdomen G beta Mmed expression levels have two peaks in stage 1 and the 5th d, but just have one peak in male abdomen in stage 1 In all other tissues expression was low and stable

分类号：

  • 相关文献

[1]Identification and Expression Pattern of Putative Odorant-Binding Proteins and Chemosensory Proteins in Antennae of the Microplitis mediator (Hymenoptera: Braconidae). Zhang, Yong-Jun,Su, Hong-Hua,Guo, Yu-Yuan,Gao, Xi-Wu.

[2]Isolation and expression analysis of glycerol-3-phosphate acyltransferase genes from peanuts (Arachis hypogaea L.). Chi, X.,Chi, X.,Pan, L.,Chen, N.,Chen, M.,Wang, T.,Wang, M.,Yang, Z.,Yu, S.,Yang, Q.,Guan, X.. 2015

[3]Binding Characterization of Recombinant Odorant-binding Proteins from the Parasitic Wasp, Microplitis mediator (Hymenoptera: Braconidae). Zhang, Shuai.

[4]Effects of Bt transgenic cotton lines on the cotton bollworm parasitoid Microplitis mediator in the laboratory. Liu, XX,Zhang, QW,Zhao, JZ,Li, HC,Xu, BL,Ma, XM. 2005

[5]Host age preference of Microplitis mediator (Hymenoptera : Braconidae), an endoparasitoid of Mythimna separata (Lepidoptera : Noctuidae). Li, Jiancheng,Coudron, Thomas A.,Pan, Wenliang,Liu, Xiaoxia,Lu, Ziyun,Zhang, Qingwen. 2006

[6]Comparative analysis of peptidoglycan recognition proteins in endoparasitoid wasp Microplitis mediator. Wang, Rui-Juan,Lu, Zhiqiang,Wang, Rui-Juan,Lin, Zhe,Jiang, Hong,Zou, Zhen,Li, Jiancheng,Lu, Ziyun,Saha, Tusar T.. 2017

[7]Gene structure and expression characteristic of a novel odorant receptor gene cluster in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae). Shan, S.,Zheng, Y.,Peng, Y.,Yang, Y. -Q.,Zhang, Y. -J.,Guo, Y. -Y.,Shan, S.,Zheng, Y.,Yang, Y. -Q.,Li, R. -J.,Lu, Z. -Y..

[8]Identification and Expression Analysis of Putative Chemosensory Receptor Genes in Microplitis mediator by Antennal Transcriptome Screening. Dhiloo, Khalid Hussain,Gu, Shao-Hua,Zhang, Yong-Jun,Guo, Yu-Yuan,Peng, Yong,Li, Rui-Jun,Lu, Zi-Yun,Zhou, Jing-Jiang,Dhiloo, Khalid Hussain. 2015

[9]Odorant Binding Characteristics of Three Recombinant Odorant Binding Proteins in Microplitis mediator (Hymenoptera: Braconidae). Wang, Shanning,Ren, Liyan,Ali, Abid,Zhang, Yongjun,Guo, Yuyuan,Li, Keming,Zhang, Kang,Zhou, Jingjiang.

[10]Seasonal cues mediate host behaviour modification and cocoon polymorphism in Microplitis mediator (Hymenoptera: Braconidae). Zhang, Jing,Liu, Xiao-Xia,Zhang, Qing-Wen,Michaud, J. P.,Li, Jian-Cheng,Luo, Shu-Ping.

[11]Cloning and expression profile of ionotropic receptors in the parasitoid wasp Microplitis mediator (Hymenoptera: Braconidae). Dhiloo, Khalid Hussain,Zheng, Yao,Shan, Shuang,Zhang, Yong-Jun,Guo, Yu-Yuan,Peng, Yong,Li, Rui-Jun,Lu, Zi-Yun,Dhiloo, Khalid Hussain,Shan, Shuang.

[12]Molecular characterization and expression profiles of olfactory receptor genes in the parasitic wasp, Microplitis mediator (Hymenoptera: Braconidae). Gu, Shao-Hua,Wang, Shan-Ning,Guo, Yu-Yuan,Zhang, Yong-Jun,Ma, Long,Liu, Ze-Wen,Zhou, Jing-Jiang. 2014

[13]Knockdown of Microplitis mediator Odorant Receptor Involved in the Sensitive Detection of Two Chemicals. Ren, Li-Yan,Zhang, Yong-Jun,Wu, Kong-Ming,Guo, Yu-Yuan,Li, Ke-Ming. 2012

[14]Role of photoperiod and temperature in diapause induction of endoparasitoid wasp Microplitis mediator (Hymenoptera : Braconidae). Li, Wenxiang,Liu, Xiaoxia,Zhang, Qingwen,Li, Wenxiang,Li, Jiancheng,Lu, Ziyun,Pan, Wenliang,Coudron, Thomas A.. 2008

[15]Field release of the parasitoid Microplitis mediator (Hymenoptera : Braconidae) for control of Helicoverpa armigera (Lepidoptera : Noctuidae) in cotton fields in Northwestern China's Xinjiang Province. Li, JC,Yan, FM,Coudron, TA,Pan, WL,Zhang, XF,Liu, XX,Zhang, QW. 2006

[16]The life parameters of a parasitoid Microplitis mediator (Hymenoptera: Braconidae), reared on cotton bollworm Helicoverpa armigera (Hubner) with Cry1Ac diet. Ding, Jun,Liu, Xiaoxia,Zhang, Qingwen,Li, Jiancheng. 2009

[17]Molecular Analysis and Expression Patterns of Four 14-3-3 Genes from Brassica napus L.. Zhan Gao-miao,Tong Jin,Wang Han-zhong,Hua Wei. 2010

[18]Genome-wide analysis of the Hsf family in soybean and functional identification of GmHsf-34 involvement in drought and heat stresses. Li, Pan-Song,Chai, Shou-Cheng,Li, Pan-Song,Yu, Tai-Fei,He, Guan-Hua,Chen, Ming,Zhou, Yong-Bin,Xu, Zhao-Shi,Ma, You-Zhi. 2014

[19]Molecular cloning and expression pattern of oriental river prawn (Macrobrachium nipponense) nitric oxide synthase. Rahman, N. M. A.,Fu, H. T.,Jin, S.,Bai, H. K.,Liang, G. X.,Gong, Y. S.,Wu, Y.,Fu, H. T.,Sun, S. M.,Qiao, H.,Jin, S.,Zhang, W. Y.,Xiong, Y. W.,Rahman, N. M. A.. 2016

[20]Characterization and Expression of Genes Encoding Three Small Heat Shock Proteins in Sesamia inferens (Lepidoptera: Noctuidae). Sun, Meng,Lu, Ming-Xing,Tang, Xiao-Tian,Du, Yu-Zhou,Sun, Meng,Lu, Ming-Xing,Tang, Xiao-Tian,Du, Yu-Zhou,Sun, Meng. 2014

作者其他论文 更多>>

微信小程序