文献类型: 外文期刊
第一作者: Zhang, Shengping
作者: Zhang, Shengping;Miao, Han;Gu, Xing-fang;Yang, Yuhong;Xie, Bingyan;Wang, Xiaowu;Huang, Sanwen;Du, Yongchen;Sun, Rifei;Wehner, Todd C.
作者机构:
关键词: Cucumis sativus;Ccu;SSR;marker-assisted selection;MAS
期刊名称:JOURNAL OF THE AMERICAN SOCIETY FOR HORTICULTURAL SCIENCE ( 影响因子:1.144; 五年影响因子:1.617 )
ISSN: 0003-1062
年卷期: 2010 年 135 卷 1 期
页码:
收录情况: SCI
摘要: Scab, caused by Cladosporium cucumerinum Ell. et Arthur, is a prevalent disease of cucumber (Cucumis sativus L.) worldwide. Scab can cause serious losses for cucumber production, especially in protected culture such as high tunnel production. Resistance to cucumber scab is dominant and is controlled by a single gene, Ccu. Breeding for resistant cultivars is the most efficient way to control the disease. Selection for resistance might be made easier if the gene were mapped to linked markers. Thus far, there are no tightly linked (genetic distance less than 1 cM) simple sequence repeat (SSR) markers for the Ccu gene, and no studies on mapping of the Ccu gene in cucumber using SSR markers. The objective of this study was to identify SSR markers for use in molecular breeding of scab resistance. In this study, we used a population of recombinant inbred lines (RILs). The population included 148 individuals derived from the cucumber inbred line 9110 Ct (Ccu Ccu) crossed with line 9930 (ccu ccu). The Ccu gene was mapped to linkage group 2, corresponding to chromosome 2 of cucumber. The flanking markers SSR03084 and SSR17631 were linked to the Ccu gene with distances of 0.7 and 1.6 cM, respectively. The veracity of SSR03084 and S5R17631 was tested using 59 diverse inbred lines and hybrids, and the accuracy rate for the two markers was 98.3%. In conclusion, two SSRs closely linked to scab resistance gene Ccu have been identified and can be used in a cucumber breeding program.
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