Molecular analysis of rice plants harboring a multi-functional T-DNA tagging system
文献类型: 外文期刊
第一作者: Ma, Yimian
作者: Ma, Yimian;Liu, Luo;Zhu, Chengguang;Sun, Changhui;Xu, Bo;Fang, Jun;Tang, Jiuyou;Luo, Anding;Cao, Shouyun;Li, Gupo;Chu, Chengcai;Ma, Yimian;Liu, Luo;Zhu, Chengguang;Sun, Changhui;Xu, Bo;Fang, Jun;Tang, Jiuyou;Luo, Anding;Cao, Shouyun;Li, Gupo;Chu, Chengcai;Ma, Yimian;Xu, Bo;Tang, Jiuyou;Qian, Qian;Xue, Yongbiao
作者机构:
关键词: rice;mutant population;promoter trap;activation tag;T-DNA integration
期刊名称:JOURNAL OF GENETICS AND GENOMICS ( 影响因子:4.275; 五年影响因子:5.223 )
ISSN: 1673-8527
年卷期: 2009 年 36 卷 5 期
页码:
收录情况: SCI
摘要: About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these Mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,3 10 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.
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