Molecular analysis of rice plants harboring a multi-functional T-DNA tagging system

文献类型: 外文期刊

第一作者: Ma, Yimian

作者: Ma, Yimian;Liu, Luo;Zhu, Chengguang;Sun, Changhui;Xu, Bo;Fang, Jun;Tang, Jiuyou;Luo, Anding;Cao, Shouyun;Li, Gupo;Chu, Chengcai;Ma, Yimian;Liu, Luo;Zhu, Chengguang;Sun, Changhui;Xu, Bo;Fang, Jun;Tang, Jiuyou;Luo, Anding;Cao, Shouyun;Li, Gupo;Chu, Chengcai;Ma, Yimian;Xu, Bo;Tang, Jiuyou;Qian, Qian;Xue, Yongbiao

作者机构:

关键词: rice;mutant population;promoter trap;activation tag;T-DNA integration

期刊名称:JOURNAL OF GENETICS AND GENOMICS ( 影响因子:4.275; 五年影响因子:5.223 )

ISSN: 1673-8527

年卷期: 2009 年 36 卷 5 期

页码:

收录情况: SCI

摘要: About 25,000 rice T-DNA insertional mutant lines were generated using the vector pCAS04 which has both promoter-trapping and activation-tagging function. Southern blot analysis revealed that about 40% of these Mutants were single copy integration and the average T-DNA insertion number was 2.28. By extensive phenotyping in the field, quite a number of agronomically important mutants were obtained. Histochemical GUS assay with 4,3 10 primary mutants revealed that the GUS-staining frequency was higher than that of the previous reports in various tissues and especially high in flowers. The T-DNA flanking sequences of some mutants were isolated and the T-DNA insertion sites were mapped to the rice genome. The flanking sequence analysis demonstrated the different integration pattern of the right border and left border into rice genome. Compared with Arabidopsis and poplar, it is much varied in the T-DNA border junctions in rice.

分类号:

  • 相关文献

[1]Phytochrome B Negatively Affects Cold Tolerance by Regulating OsDREB1 Gene Expression through Phytochrome Interacting Factor-Like Protein OsPIL16 in Rice. He, Yanan,Li, Yaping,Cui, Lixin,Xie, Lixia,Zheng, Chongke,Zhou, Guanhua,Zhou, Jinjun,Xie, Xianzhi,Li, Yaping,Cui, Lixin. 2016

[2]A Kelch Motif-Containing Serine/Threonine Protein Phosphatase Determines the Large Grain QTL Trait in Rice. Hu, Zejun,Sun, Fan,Xin, Xiaoyun,Qian, Xi,Yang, Jingshui,Luo, Xiaojin,Hu, Zejun,He, Haohua,Wang, Wenxiang,Zhang, Shiyong. 2012

[3]Genome-wide identification of microRNAs and their targets in wild type and phyB mutant provides a key link between microRNAs and the phyB-mediated light signaling pathway in rice. Sun, Wei,wu, Xiu,Xie, Xianzhi,Xu, Xiao Hui,Lu, Xingbo,Sun, Hongwei,Wang, Yong. 2015

[4]Overexpression of an S-like ribonuclease gene, OsRNS4, confers enhanced tolerance to high salinity and hyposensitivity to phytochrome-mediated light signals in rice. Zheng, Jun,Wang, Yingying,He, Yanan,Zhou, Jinjun,Li, Yaping,Liu, Qianqian,Xie, Xianzhi,Zheng, Jun,Wang, Yingying,He, Yanan,Zhou, Jinjun,Xie, Xianzhi. 2014

[5]Distribution Characteristics of Soil Cadmium in Different Textured Paddy Soil Profiles and Its Relevance with Cadmium Uptake by Crops. Wang Zheng-yin,Qin Yu-sheng,Zhan Shao-jun,Yu Hua,Tu Shi-hua. 2013

[6]Overexpression of OsPIL15, a phytochromeinteracting factor- like protein gene, represses etiolated seedling growth in rice. Zhou, Jinjun,Liu, Qianqian,Wang, Yingying,Zhang, Shiyong,Cheng, Huimin,Yan, Lihua,Li, Li,Xie, Xianzhi,Zhou, Jinjun,Wang, Yingying,Zhang, Shiyong,Xie, Xianzhi,Liu, Qianqian,Xie, Xianzhi,Zhang, Fang,Chen, Fan. 2014

[7]DISTRIBUTION CHARACTERISTICS, BIOACCUMULATION, AND SOURCES OF MERCURY IN RICE AT NANSI LAKE AREA, SHANDONG PROVINCE, CHNIA. Liu, H.,Zhang, J.,Dai, J. L.,Wang, L. H.,Zhang, J.,Li, G. X.. 2015

[8]Nitrogen Status Diagnosis of Rice by Using a Digital Camera. Fan Ming-sheng,Zhang Fu-suo,Chen Xin-ping,Jia Liang-liang,Sun Yan-ming,Lue Shi-hua. 2009

[9]Influence of unflooded mulching cultivation on nitrogen uptake and utilization of fertilizer nitrogen by rice. Liu, Xuejun,Zhang, Fusuo,Mao, Daru,Zeng, Xingzhong,Lu, Shihua,Wang, Mingtian. 2008

[10]Overexpression of a phytochrome-regulated tandem zinc finger protein gene, OsTZF1, confers hypersensitivity to ABA and hyposensitivity to red light and far-red light in rice seedlings. Zhou, Jinjun,Fan, Zhongxue,Xie, Xianzhi,Zhang, Cheng,Zhou, Jinjun,Fan, Zhongxue,Xie, Xianzhi,Zhang, Cheng,Ma, Huiquan,Zhang, Fang,Chen, Fan. 2012

[11]Enhancement of innate immune system in monocot rice by transferring the dicotyledonous elongation factor Tu receptor EFR. Lu, Fen,Wang, Huiqin,Wang, Shanzhi,Jiang, Wendi,Yang, Jun,Sun, Wenxian,Lu, Fen,Wang, Huiqin,Wang, Shanzhi,Jiang, Wendi,Yang, Jun,Sun, Wenxian,Shan, Changlin,Li, Bin,Shan, Changlin,Li, Bin,Yang, Jun,Zhang, Shiyong. 2015

[12]A missense mutation in the transmembrane domain of CESA9 affects cell wall biosynthesis and plant growth in rice. Wang, Daofeng,Lan, Jinhao,Wang, Daofeng,Zhao, Jinfeng,Li, Xueyong,Yuan, Shoujiang,Yin, Liang,Guo, Baotai. 2012

[13]Mutations in the MIT3 gene encoding a caroteniod isomerase lead to increased tiller number in rice. Liu, Lihua,Peng, Peng,Qiu, Haiyang,Zhao, Jinfeng,Fang, Jingjing,Patil, Suyash Bhimgonda,Li, Xueyong,Xie, Tingting,Zhang, Wenhui,Wang, Yiqin,Fang, Shuang,Chu, Jinfang,Yuan, Shoujiang. 2018

[14]Detection of epistatic interactions of three QTLs for heading date in rice using single segment substitution lines. Ding, Han-Feng,Liu, Xu,Li, Run-Fang,Wang, Wen-Ying,Zhang, Y.,Zhang, Xiao-Dong,Yao, Fang-Yin,Li, Guang-Xian,Jiang, Ming-Song,Ding, Han-Feng.

[15]Delimitation of the PSH1(t) gene for rice purple leaf sheath to a 23.5 kb DNA fragment. Wang, Wen-Ying,Ding, Han-Feng,Li, Run-Fang,Liu, Xu,Zhang, Yu,Yao, Fang-Yin,Li, Guang-Xian,Jiang, Ming-Song.

[16]Determination of 5-hydroxymethyl-2-deoxycytidine in Rice by High-performance Liquid Chromatography-Tandem Mass Spectrometry with Isotope Dilution. Wang, Xiaoli,Wang, Shanshan,Chen, Xiangfeng,Chen, Yue,Yuan, Jinpeng,Zhao, Rusong,Guo, Tao. 2017

[17]Functional Marker Development and Effect Analysis of Grain Size Gene GW2 in Extreme Grain Size Germplasm in Rice. Zhang Ya-dong,Zheng Jia,Liang Yan-li,Zhao Chun-fang,Chen Tao,Zhao Qing-yong,Zhu Zhen,Zhou Li-hui,Yao Shu,Zhao Ling,Yu Xing,Wang Cai-lin. 2015

[18]Synonymous codon usage and gene function are strongly related in Oryza sativa. Liu, QP,Dou, SJ,Ji, ZJ,Xue, QZ. 2005

[19]Correlation between appearance of embryogenic cells and the IAA levels in rice somatic cell culture. Chen, YF,Zhou, X,Tang, RS,Zhang, JY,Mei, CS. 1998

[20]Disruption of Secondary Wall Cellulose Biosynthesis Alters Cadmium Translocation and Tolerance in Rice Plants. Song, Xue-Qin,Liu, Li-Feng,Zhang, Bao-Cai,Gao, Ya-Ping,Liu, Xiang-Ling,Zhou, Yi-Hua,Jiang, Yi-Jun,Lin, Qing-Shan,Ling, Hong-Qing. 2013

作者其他论文 更多>>