Carbamoyl Phosphate Synthase Subunit CgCPS1 Is Necessary for Virulence and to Regulate Stress Tolerance in Colletotrichum gloeosporioides

文献类型： 外文期刊

第一作者： Mushtaq, Aamar

作者： Mushtaq, Aamar;Tariq, Muhammad;Ahmed, Maqsood;Ali, Imran;Mahmood, Raja Tahir;Mushtaq, Aamar;Zhou, Zongshan

作者机构：

关键词： apple; Colletotrichum gloeosporioides; gene knockout; Glomerella leaf spot; virulence

期刊名称：PLANT PATHOLOGY JOURNAL （ 影响因子：1.795； 五年影响因子：2.314 ）

ISSN： 1598-2254

年卷期： 2021 年 37 卷 3 期

页码：

收录情况： SCI

摘要： Glomerella leaf spot (GLS) is a severe infectious disease of apple whose infective area is growing gradually and thus poses a huge economic threat to the world. Different species of Colletotrichum including Colletotrichum gloeosporioides are responsible for GLS. For efficient GLS control, it is important to understand the mechanism by which the cruciferous crops and C. gloeosporioides interact. Arginine is among one of the several types of amino acids, which plays crucial role in biochemical and physiological functions of fungi. The arginine biosynthesis pathway involved in virulence among plant pathogenic fungi is poorly understood. In this study, CgCPS1 gene encoding carbamoyl phosphate synthase involved in arginine biosynthesis has been identified and inactivated experimentally. To assess the effects of CgCPS1, we knocked out CgCPS1 in C. gloeosporioides and evaluated its effects on virulence and stress tolerance. The results showed that deletion of CgCPS1 resulted in loss of pathogenicity. The Acgcps1 mutants showed slow growth rate, defects in appressorium formation and failed to develop lesions or apple leaves and fruits leading to loss of virulence while complementation strain (CgCPS1-C) fully restored its pathogenicity. Furthermore, mutant strains showed extreme sensitivity to high osmotic stress displaying that CgCPS1 plays a vital role in stress response. These findings suggest that CgCPS1 is major factor that mediates pathogenicity in C. gloeosporioides by encoding carbamoyl phosphate that is involved in arginine biosynthesis and conferring virulence in C. gloeosporioides.

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