Reverse genetic manipulation of the overlapping coding regions for structural proteins of the type II porcine reproductive and respiratory syndrome virus
文献类型: 外文期刊
第一作者: Yu, Dandan
作者: Yu, Dandan;Lv, Jian;Sun, Zhi;Zheng, Haihong;Lu, Jiaqi;Yuan, Shishan
作者机构:
关键词: PRRSV;Structural protein;Overlapping coding region;Reverse genetic manipulation;Transcription regulating sequence;TRS
期刊名称:VIROLOGY ( 影响因子:3.616; 五年影响因子:3.967 )
ISSN: 0042-6822
年卷期: 2009 年 383 卷 1 期
页码:
收录情况: SCI
摘要: The overlapping genomic regions coding for structural proteins of porcine reproductive and respiratory syndrome virus (PRRSV) poses problems for molecular dissection of the virus replication process. We constructed five mutant full-length cDNA clones with the overlapping regions unwound and 1 to 3 restriction sites inserted between two adjacent ORFs (ORF1/2, ORF4/5, ORF5/6, ORF 6/7 and ORF7/3' UTR), which generated the recombinant viruses. Our findings demonstrated that 1) the overlapping structural protein ORFs can be physically separated, and is dispensable for virus viability; 2) Such ORF separations did not interrupt the subgenomic RNA synthesis; 3) the plaque morphology, growth kinetics, and antigenicity of these mutant viruses were virtually indistinguishable from those of the parental virus in cultured cells; and 4) these mutant viruses remained genetic stable in vitro. This study lays a foundation for further molecular dissection of PRRSV replication process, and development of genetically tagged vaccines against PRRS. (c) 2008 Elsevier Inc. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Nitazoxanide synergizes polymyxin B against Escherichia coli by depleting cellular energy
作者:Jiang, Xuejia;Chen, Dongliang;Wang, Xiaoyang;Wang, Chunmei;Zheng, Haihong;Ye, Wenchong;Zhou, Wen;Zhang, Keyu;Liu, Guoping
关键词:antibiotic adjuvant; nitazoxanide; antibiotic resistance; gram-negative bacteria; polymyxin B
-
Targeting TRAF7 to Enhance Angiogenesis and Mitigate Ischemia-Induced Brain Damage: Insights from in Vivo and in Vitro Models
作者:Feng, Nianping;Wu, Yun;Li, Genjie;Li, Yushan;Guan, Yuexin;Xiao, Xingjun;Zheng, Haihong;Ji, Jingjing;Zhang, Zhuo
关键词:cerebral ischemic stroke; angiogenesis; TRAFA7; KLF4; VEGF
-
Natural variation in Fatty Acid 9 is a determinant of fatty acid and protein content
作者:Qi, Zhaoming;Li, Hui;Jong, CholNam;Zhang, Yu;Hu, Limin;Wu, Xiaoxia;Xin, Dawei;Yang, Mingliang;Liu, Chunyan;Chen, Qingshan;Guo, Chaocheng;Yu, Guolong;Wang, Xu;Li, Haiyang;Kong, Fanjiang;Qiu, Hongmei;Lv, Jian
关键词:Soybean; Seed fatty acid content; Seed protein content; Natural variation; Domestication
-
The modulation of metabolomics and antioxidant stress is involved in the effect of nitazoxanide against influenza A virus in vitro
作者:Huang, Zhen;Zheng, Haihong;Wang, Yanping;Wang, Xiaoyang;Wang, Chunmei;Liu, Yingchun;Zhou, Wen;Zhang, Keyu;Huang, Zhen;Wang, Zhaoxiong
关键词:nitazoxanide; influenza A virus; metabolomics; anti-oxidative stress; Nrf2
-
The low ferric chelate reductase activity and high apoplastic pH in leaves cause iron deficiency chlorosis in 'Huangguan' pears grafted onto quince A grown in calcareous soil
作者:Zhao, Yanyan;Li, Fei;Sun, Zhi;Li, Haigang;Zhao, Yanyan;Liu, Songzhong;Sun, Mingde;Liang, Zhenxu;Yu, Futong
关键词:Pears; Quince A; Iron deficiency; Calcareous soil; Apoplastic pH
-
A highly efficient indirect ELISA and monoclonal antibody established against African swine fever virus pK205R
作者:Li, Liwei;Qiao, Sina;Liu, Jiachen;Zhou, Yanjun;Tong, Wu;Liu, Changlong;Jiang, Yifeng;Guo, Ziqiang;Zheng, Haihong;Tong, Guangzhi;Li, Guoxin;Gao, Fei;Qiao, Sina;Dong, Shishan;Zhao, Ran;Li, Guoxin;Gao, Fei
关键词:ASFV pK205R; 293i cells; epitope; indirect ELISA; recombinant PRRSV
-
PSMB1 Inhibits the Replication of Porcine Reproductive and Respiratory Syndrome Virus by Recruiting NBR1 To Degrade Nonstructural Protein 12 by Autophagy
作者:Li, Liwei;Bai, Yuanzhe;Zhou, Yanjun;Jiang, Yifeng;Tong, Wu;Li, Guoxin;Zheng, Haihong;Gao, Fei;Tong, Guangzhi;Li, Liwei;Gao, Fei;Tong, Guangzhi
关键词:PSMB1; PRRSV; Nsp12; NBR1; autophagic degradation