Identification of SSR markers for a broad-spectrum blast resistance gene Pi20(t) for marker-assisted breeding

文献类型: 外文期刊

第一作者: Wan, Jianmin

作者: Wan, Jianmin;Li, Wei;Jia, Yulin

作者机构:

关键词: gene mapping;Magnaporthe oryzae;marker-assisted selection;Oryza sativa l;resistance gene;rice blast

期刊名称:MOLECULAR BREEDING ( 影响因子:2.589; 五年影响因子:2.75 )

ISSN: 1380-3743

年卷期: 2008 年 22 卷 1 期

页码:

收录情况: SCI

摘要: The Pi20(t) gene was determined to confer a broad-spectrum resistance against diverse blast pathotypes (races) in China based on inoculation experiments utilizing 160 Chinese Magnaporthe oryzae (formerly Magnaporthe grisea) isolates, among which isolate 98095 can specifically differentiate the Pi20(t) gene present in cv. IR24. Two flanking and three co-segregating simple sequence repeat (SSR) markers for Pi20(t), located near the centromere region of chromosome 12, were identified using 526 extremely susceptible F(2) plants derived from a cross of Asominori, an extremely susceptible cultivar, with resistant cultivar IR24. The SSR OSR32 was mapped at a distance of 0.2 cM from Pi20(t), and the SSR RM28050 was mapped to the other side of Pi20(t) at a distance of 0.4 cM. The other three SSR markers, RM1337, RM5364 and RM7102, co-segregated with Pi20(t). RM1337 and RM5364 were found to be reliable markers of resistance conditioned by Pi20(t) in a wide range of elite rice germplasm in China. As such, they are useful tags in marker-assisted rice breeding programs aimed at incorporating Pi20(t) into advanced rice breeding lines and, ultimately, at obtaining a durable and broad spectrum of resistance to M. oryaze.

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