Production and characterization of intertribal somatic hybrids of Raphanus sativus and Brassica rapa with dye and medicinal plant Isatis indigotica

文献类型： 外文期刊

第一作者： Tu, Yuqin

作者： Tu, Yuqin;Sun, Jian;Liu, Yan;Ge, Xianhong;Zhao, Zhigang;Yao, Xingcheng;Li, Zaiyun;Sun, Jian

作者机构：

关键词： Isatis indigotica;Raphanus sativus;Brassica rapa;intertribal hybrids;genomic in situ hybridization (GISH)

期刊名称：PLANT CELL REPORTS （ 影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2008 年 27 卷 5 期

页码：

收录情况： SCI

摘要： Intertribal somatic hybrids of Raphanus sativus (2n = 18, RR) and Brassica rapa spp. chinensis (2n = 20, AA) with the dye and medicinal plant Isatis indigotica (2n = 14, I I) were firstly obtained by polyethylene glycol-induced symmetric fusions of mesophyll protoplasts. One mature hybrid with R. sativus established in field had intermediate morphology but was totally sterile. It had the expected chromosome number (2n = 32, RRI I) and parental chromosomes were distinguished by genomic in situ hybridization (GISH) analysis, and these chromosomes were paired as 16 bivalents in pollen mother cells (PMCs) at diakinesis and mainly segregated equally as 16:16 at anaphase I (A I), but the meiotic disturbance in second division was obvious. Five mature hybrids with B. rapa established in field were morphologically intermediate but showed some differences in phenotypic traits and fertility, two were partially fertile. Cytological and GISH investigations revealed that these hybrids had 2n = 48 with AAIIII complement and their PMCs showed normal pairing of 24 bivalents and mainly equal segregation 24:24, but meiotic abnormalities of lagging chromosomes and micronuclei appeared frequently during second divisions. AFLP analysis showed that all of these hybrids had mainly the DNA banding pattern from the addition of two parents plus some alterations. Some hybrids should be used for the genetic improvement of crops and the dye and medicinal plant.

分类号：

  • 相关文献

[1]Plant regeneration capacity of mesophyll protoplasts from Brassica napus and related species. Hu, Q,Andersen, SB,Hansen, LN. 1999

[2]Allelopathic effects of Hemistepta lyrata on the germination and growth of wheat, sorghum, cucumber, rape, and radish seeds. Gao, Xingxiang,Li, Mei,Gao, Zongjun,Li, Changsong,Sun, Zuowen.

[3]Production and characterization of intergeneric hybrids between turnip (Brassica rapa L. em. Metzg. subsp rapa) and radish (Raphanus sativus L.). Lou, Lina,Xu, Yuanyuan,Liu, Zhe,Su, Xiaojun,Lou, Qunfeng,Li, Ziang.

[4]Cloning and characterization of a novel sulfate transporter gene from radish (Raphanus sativus L.). Wang, Zhi-Wei,Xiang, Chang-Ping,Mei, Shi-Yong.

[5]Identification of Cytoplasmic Male Sterility in Chinese Radish Following PCR Analysis of Mitochondrial DNA. Zhang, Li,Yasumoto, Keita,Yamagishi, Hiroshi.

[6]Chromosomal assignment of oil radish resistance to Meloidogyne incognita and M. javanica using a set of disomic rapeseed-radish chromosome addition lines. Zhang, Shaosong,Schliephake, Edgar,Budahn, Holger.

[7]Genetic and cytological analysis of a new spontaneous male sterility in radish (Raphanus sativus L.). Wang, Zhi-Wei,Gao, Lei,Zhou, Yuan,Wang, Ting,Wang, Zhi-Wei,Xiang, Chang Ping,Liu, Hai Zhou,Mei, Shi Yong.

[8]Molecular characterization of a Thinopyrum intermedium Group 2 chromosome (2Ai-2) conferring resistance to barley yellow dwarf virus. Zhang, ZY,Xin, ZY,Larkin, PJ. 2001

[9]Identification of molecular markers for the Thinopyrum intermedium chromosome 2Ai-2 with resistance to barley yellow dwarf virus. Zhang, ZY,Xin, ZY,Lin, ZS,Chen, X,Wang, XP. 2000

[10]Chromosomal distribution of the 18S-5.8S-26S rDNA loci and heterogeneity of nuclear in regions in Thinopyrum intermedium (Poaceae : Triticeae). Li, DY,Ru, YY,Zhang, XY. 2004

[11]Primary identification of alien chromatin in T911289, a maintainer of wheat male sterile line with cytoplasm of Aegilops kotschyi. Liu, BS,Li, DY,Zhang, XY,Gao, QR,Sun, LZ,Sun, QX,Dong, ST. 2003

[12]Identification of Wheat-Barley 2H Alien Substitution Lines. Yuan, YP,Chen, X,Xiao, SH,Islam, AKMR,Xin, ZY.

[13]Chromosomal Distribution of the 18S-5.8S-26S rDNA Loci and Heterogeneity of Nuclear ITS Regions in Thinopyrum intermedium (Poaceae: Triticeae). Li Dayong,Ru Yanyan,Zhang Xueyong. 2004

[14]Molecular cytogenetic identification of a wheat-Thinopyron intermedium (Host) Barkworth & DR Dewey partial amphiploid resistant to powdery mildew. Wang, HG,Zhang, XY,Li, XF,Li, DY,Duan, XY,Zhou, YL. 2005

[15]Characterization of euploid backcross progenies derived from interspecific hybrids between Oryza sativa and O-eichingeri by restriction fragment length polymorphism (RFLP) analysis and genomic in situ hybridization (GISH). Yan, HH,Liu, GQ,Cheng, ZK,Min, SK,Zhu, LH. 2001

[16]Genetic relationship and genomic in situ hybridization analysis of the three genomes in Triticum aestivum. Li, DY,Zhang, XY,Yang, J,Rao, GY. 2000

[17]Cytogenetic Identification of a New Hexaploid Coix aquatica Cyto-type. Li, DY,Li, YC,Xue, YG,Hu, ZL,Song, YC.

[18]Production and characterization of interspecific somatic hybrids between Brassica oleracea var. botrytis and B. nigra and their progenies for the selection of advanced pre-breeding materials. Wang, Gui-xiang,Tang, Yu,Sheng, Xiao-guang,Hao, Wei-wei,Zhang, Li,Lu, Kun,Liu, Fan,Yan, Hong. 2011

[19]Trichokonins from Trichoderma pseudokoningii SMF2 induce resistance against Gram-negative Pectobacterium carotovorum subsp carotovorum in Chinese cabbage. Li, Hai-Yun,Luo, Yan,Shi, Wei-Ling,Gong, Zhi-Ting,Shi, Mei,Chen, Xiu-Lan,Zhang, Yu-Zhong,Song, Xiao-Yan,Li, Hai-Yun,Zhang, Xiu-Sheng,Chen, Lei-Lei. 2014

[20]Molecular characterization of novel haplotypes of eIF4E family in Chinese cabbage (Brassica rapa L. ssp pekinensis). Liu, Shuan-Tao,Zhang, Zhi-Gang,Li, Qiao-Yun,Wang, Shu-Fen,Zhao, Zhi-Zhong,Lu, Jin-Dong,Xu, Wen-Ling,Liu, Xian-Xian,Fu, Wei-Min. 2013

作者其他论文 更多>>

微信小程序