Studies on Agrobacterium-mediated genetic transformation of embryogenic suspension cultures of sweet potato
文献类型: 外文期刊
第一作者: Xing, Yu-Jun
作者: Xing, Yu-Jun;Ji, Qin;Luo, Yu-Ming;Xing, Yu-Jun;Yang, Qing;Li, Qiang;Wang, Xin
作者机构:
关键词: ipomoea batatas;Agrobacterium-mediated transformation;SBD2 gene;embryogenesis
期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )
ISSN: 1684-5315
年卷期: 2008 年 7 卷 5 期
页码:
收录情况: SCI
摘要: In this study, genetic transformation of embryogenic suspension cultures of sweet potato (Ipomoea batatas) cultivar Xu55-2 was conducted utilizing the Agrobacterium tumefaciens strain EHA105 that contains the binary vector pBIN19/SBD2 with SBD2 (starch binding domain 2) gene and neomycin phosphotransferase (NPT II) gene. The presence of the SBD2 gene in the genomic DNA of transgenic plants was verified by PCR amplification and confirmed by Southern blot analysis. Results suggested that cefotaxime (Cefo), at the concentration of 200 mg/L, was able to effectively suppress the growth of Agrobacterium after co-cultivation. The optimal concentration for kanamycin (Kan) was 10 mg/L for selecting resistance calli, somatic embryo formation and plant regeneration. The highest frequency of shoot induction (30.9%) was obtained on the MS medium containing 10 mg/L Kan, 200 mg/L Cefo, 1.0 mg/L abscisic acid (ABA) and 1.0 mg/L gibberellic acid (GA(3)).
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