Studies on Agrobacterium-mediated genetic transformation of embryogenic suspension cultures of sweet potato

文献类型: 外文期刊

第一作者: Xing, Yu-Jun

作者: Xing, Yu-Jun;Ji, Qin;Luo, Yu-Ming;Xing, Yu-Jun;Yang, Qing;Li, Qiang;Wang, Xin

作者机构:

关键词: ipomoea batatas;Agrobacterium-mediated transformation;SBD2 gene;embryogenesis

期刊名称:AFRICAN JOURNAL OF BIOTECHNOLOGY ( 影响因子:0.573; 五年影响因子:0.794 )

ISSN: 1684-5315

年卷期: 2008 年 7 卷 5 期

页码:

收录情况: SCI

摘要: In this study, genetic transformation of embryogenic suspension cultures of sweet potato (Ipomoea batatas) cultivar Xu55-2 was conducted utilizing the Agrobacterium tumefaciens strain EHA105 that contains the binary vector pBIN19/SBD2 with SBD2 (starch binding domain 2) gene and neomycin phosphotransferase (NPT II) gene. The presence of the SBD2 gene in the genomic DNA of transgenic plants was verified by PCR amplification and confirmed by Southern blot analysis. Results suggested that cefotaxime (Cefo), at the concentration of 200 mg/L, was able to effectively suppress the growth of Agrobacterium after co-cultivation. The optimal concentration for kanamycin (Kan) was 10 mg/L for selecting resistance calli, somatic embryo formation and plant regeneration. The highest frequency of shoot induction (30.9%) was obtained on the MS medium containing 10 mg/L Kan, 200 mg/L Cefo, 1.0 mg/L abscisic acid (ABA) and 1.0 mg/L gibberellic acid (GA(3)).

分类号:

  • 相关文献

[1]Establishment of a grafted overhead-sweetpotato cultivation system with root-function spatial division. Peng, Meifang,Pu, Zhigang,Yan, Wenzhao,Fu, Yufan,Tan, Wenfang,Wang, Dayi.

[2]Leaf Spot on Sweet Potato (Ipomoea batatas) Caused by Stemphylium solani, a New Disease in China. Chai, A-Li,Du, Gong-Fu,Shi, Yan-Xia,Xie, Xue-Wen,Li, Bao-Ju.

[3]IDENTIFICATION OF miRNAs IN SWEET POTATO BY SOLEXA SEQUENCING. Bian, X.,Ma, P.,Jia, Z.,Guo, X.,Xie, Y.,E, Z..

[4]Efficient elimination of sweetpotato little leaf phytoplasma from sweetpotato by cryotherapy of shoot tips. Valkonen, J. P. T.,Wang, Q. C..

[5]Cloning and characterization of a novel GIGANTEA gene in sweet potato. Tang, Wei,Yan, Hui,Su, Zai-xing,Park, Sung-Chul,Liu, Ya-ju,Zhang, Yun-gang,Wang, Xin,Kou, Meng,Ma, Dai-fu,Li, Qiang,Park, Sung-Chul,Kwak, Sang-Soo. 2017

[6]Sweetpotato viruses in China. Wang, Qingmei,Zhang, Liming,Wang, Biao,Yin, Zhenfang,Feng, Chaohong,Wang, Qiaochun.

[7]Small RNA profiling and degradome analysis reveal regulation of microRNA in peanut embryogenesis and early pod development. Gao, Chao,Wang, Pengfei,Zhao, Shuzhen,Zhao, Chuanzhi,Xia, Han,Hou, Lei,Zhang, Ye,Li, Changsheng,Wang, Xingjun,Wang, Xingjun,Ju, Zheng. 2017

[8]Genome-wide analysis of WOX genes in upland cotton and their expression pattern under different stresses. Zhaoen Yang,Qian Gong,Wenqiang Qin,Qin, Wenqiang,Yang, Zhaoen,Zuoren Yang,Yuan Cheng,Lili Lu,Xiaoyang Ge,Chaojun Zhang,Zhixia Wu,Fuguang Li. 2017

[9]Plant development from microspore-derived embryos in oilseed rape as affected by chilling, desiccation and cotyledon excision. Zhang, GQ,Zhang, DQ,Tang, GX,He, Y,Zhou, WJ. 2006

[10]High frequency spontaneous production of doubled haploid plants in microspore cultures of Brassica rapa ssp chinensis. Gu, HH,Zhou, WJ,Hagberg, P. 2003

[11]Effects of pH, MES, arabinogalactan-proteins on microspore cultures in white cabbage. Yuan, Su-xia,Su, Yan-bin,Liu, Yu-mei,Fang, Zhi-yuan,Yang, Li-mei,Zhuang, Mu,Zhang, Yang-yong,Sun, Pei-tian.

[12]Improvement of Agrobacterium-mediated transformation efficiency and transgenic plant regeneration of Vitis vinifera L. by optimizing selection regimes and utilizing cryopreserved cell suspensions. Li, P,Hanania, U,Sahar, N,Mawassi, M,Gafny, R,Sela, I,Tanne, E,Perl, A.

[13]Cloning, molecular characterization and expression analysis of heat shock cognate 70 (Hsc70) cDNA from turbot (Scophthalmus maximus). Wang, T. T.,Wang, N.,Liao, X. L.,Meng, L.,Liu, Y.,Chen, S. L.,Wang, T. T..

[14]Cloning, genomic structure and expression analysis of ubc9 in the course of development in the half-smooth tongue sole (Cynoglossus semilaevis). Hu, Qiaomu,Chen, Songlin,Hu, Qiaomu.

[15]Effect of combined cold pretreatment and heat shock on microspore cultures in broccoli. Yuan, S. X.,Liu, Y. M.,Fang, Z. Y.,Yang, L. M.,Zhuang, M.,Zhang, Y. Y.,Sun, P. T..

[16]Hunchback IS REQUIRED FOR ABDOMINAL IDENTITY SUPPRESSION AND GERMBAND GROWTH IN THE PARTHENOGENETIC EMBRYOGENESIS OF THE PEA APHID, Acyrthosiphon pisum. Liu, Changyan,Zeng, Fanrong.

[17]Improvement of isolated microspore culture of barley (Hordeum vulgare L.): the effect of floret co-culture. Lu, Ruiju,Wang, Yifei,Sun, Yuefang,Shan, Lili,Huang, Jianhua,Lu, Ruiju,Wang, Yifei,Sun, Yuefang,Shan, Lili,Huang, Jianhua,Lu, Ruiju,Chen, Peidu.

[18]Anther culture and plant regeneration of cotton (Gossypium klotzschianum Anderss). Feng, R,Li, XH,Li, FL. 1996

[19]Morphological anatomy, sporogenesis and gametogenesis in flowering process of jasmine (Jasminum sambac Aiton). Deng, Yanming,Jia, Xinping,Liang, Lijian,Sun, Xiaobo,Gu, Chunsun.

[20]Induction and development of microspore-derived embryos in broccoli x white-headed cabbage hybrids microspore culture. Zeng, Aisong,Song, Lixiao,Liu, Huanhuan,Hou, Xilin,Li, Ying,Zeng, Aisong,Yan, Jiyong,Song, Lixiao,Gao, Bing,Zhang, Yunxia,Li, Jianqi.

作者其他论文 更多>>