Complete nucleotide sequence of a Chinese serotype Asia1 vaccine strain of foot-and-mouth disease virus

文献类型: 外文期刊

第一作者: Du, Junzheng

作者: Du, Junzheng;Chang, Huiyun;Cong, Guozheng;Shao, Junjun;Lin, Tong;Shang, Youjun;Liu, Zaixin;Liu, Xiangtao;Cai, Xuepeng;Me, Qingge

作者机构:

关键词: foot-and-mouth disease virus;asia1/YNBS/58;genome;phylogenetic tree

期刊名称:VIRUS GENES ( 影响因子:2.332; 五年影响因子:2.0 )

ISSN: 0920-8569

年卷期: 2007 年 35 卷 3 期

页码:

收录情况: SCI

摘要: The full-length nucleotide sequence of the Chinese vaccine strain Asia1/YNBS/58 of foot-and-mouth disease virus (FMDV) was determined. The results showed that the complete genome of YNBS/58 is 8,164 nucleotides (nt) in length including a 1,061-nt 5' untranslated region (UTR),a 6,990-nt open reading frame (ORF), and a 113-nt 3' UTR.. Genome sequences of Asial/YNBS/58 and other known FMDV strains were compared. The homology analysis indicated that non-structural proteins are more conserved than structural proteins in FMDV and that the 5'UTR is more conserved than the 3' UTR. Phylogenetic analysis revealed that Asial/YNBS/58 is clustered in the Asial serotype and is linked to three other isolates, Asia1/IND/63/72, Asia1/ 3kimron/63, and Asia1/2ISRL/63, suggesting that they have a close genetic relationship. The VP1-, VP2-, and VP3-based phylogenetic trees divided into distinct clusters according to the different serotypes, while other gene-based phylogenetic trees exhibited some degree of intercrossing among serotypes. According to the nucleotide similarities between Asial/YNBS/58 and two recent Chinese Asial isolates, Asia1/HKN/05, and Asia1/JS/05, each forms a distinct genotype. This study is the first description of the full-length genomic sequence of FMDV Chinese serotype Asia1.

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