Comparison of gene expression patterns between porcine cumulus-oocyte complexes and naked oocytes

文献类型： 外文期刊

第一作者： Zhu, G. Y.

作者： Zhu, G. Y.;Feng, S. T.;Li, J. T.;Mu, Y. L.;Pan, D. K.;Guo, B. R.

作者机构：

关键词： differential gene expression;DD-RT-PCR;porcine oocytes;cumulus

期刊名称：SOUTH AFRICAN JOURNAL OF ANIMAL SCIENCE （ 影响因子：1.055； 五年影响因子：1.249 ）

ISSN： 0375-1589

年卷期： 2007 年 37 卷 1 期

页码：

收录情况： SCI

摘要： Several layers of cumulus cells surround the oocyte in the antral ovarian follicle throughout the follicular development until ovulation. The cumulus cells play an important supportive and regulative role in oocyte development and maturation via intercellular communications between oocytes and cumulus cells. Using the differential display reverse transcriptase polymerase chain reaction (DD-RT-PCR) and semiquantitative RT-PCR methods, we compared the mRNA expression patterns in porcine oocytes from two sources: cumulus-oocyte complexes (COCs) and naked oocytes (NOs). After a small scale screen, 14 differentially expressed mRNAs were cloned, sequenced, and their expression patterns were verified by semi-quantitative RT-PCR. It was confirmed that four mRNAs including three genes (PELPI, Myo5b and CAST) and a new EST (Expressed Sequence Tag) are expressed preferentially in the oocytes of COCs rather than in NOs. Previous studies of the three genes suggested that they are mainly involved in oestrogen receptor regulation, membrane trafficking, organelle transport, cellular signalling and some other cellular processes. These results suggest that the aberrant of gene expression patterns detected in the oocytes of NOs compared with COCs explains their reduced quality in terms of development and rnaturation. In conclusion, these differentially expressed mRNAs may be involved in cellular interactions between oocytes and cumulus cells and thus could be considered as essential genes for the competence of oocytes.

分类号：

  • 相关文献

[1]Expression of bone morphogenetic proteins and receptors in porcine cumulus-oocyte complexes during in vitro maturation. Zhu, Guiyu,Pan, Dengke,Mu, Yulian,Feng, Shutang,Zhu, Guiyu,Guo, Bingran. 2008

[2]Predictive value of the area of expanded cumulus mass on development of porcine oocytes matured and fertilized in vitro. Qian, Y,Shi, WQ,Ding, JT,Sha, JH,Fan, BQ.

[3]A new tool for in vitro culture of porcine eggs. Zheng, Xin-Min,Qiao, Xian-Feng,Wei, Qing-Xin,Xiao, Hong-Wei,Liu, Xi-Mei,Li, Li,Hua, Zai-Dong,Xu, Hou-Qiang. 2011

[4]Subcellular Characterization of Porcine Oocytes with Different Glucose-6-phosphate Dehydrogenase Activities. Fu, Bo,Liu, Di,Ma, Hong,Zhang, Dong-Jie,Guo, Zhen-Hua,Fu, Bo,Ma, Jian-Zhang,An, Tie-Zhu,Ren, Liang,Liu, Di,Yang, Xiu-Qin,Guo, Yun-Yun,Zhu, Meng,Bai, Jing.

[5]POSITIVE EFFECT OF APOPTOTIC INHIBITOR Z-VAD-FMK ON VITRIFIED-THAWED PORCINE MII STAGE OOCYTES. Niu, Yingfang,Dai, Jianjun,Chen, Yaning,Wu, Caifeng,Zhang, Shushan,Zhang, Defu. 2016

[6]MC1568 Enhances Histone Acetylation During Oocyte Meiosis and Improves Development of Somatic Cell Nuclear Transfer Embryos in Pig. Wang, Huili,Meng, Chunhua,Zhang, Jun,Li, Yinxia,Qian, Yong,Xing, Guangdong,Cao, Shaoxian,Cui, Wei,Zhao, Dongmin. 2018

[7]Development of porcine tetraploid somatic cell nuclear transfer embryos is influenced by oocyte nuclei. Fu, Bo,Liu, Di,Ma, Hong,Guo, Zhen-Hua,Wang, Liang,Li, Zhong-Qiu,Peng, Fu-Gang,Liu, Di,Bai, Jing.

[8]Relationship Between Gene Differential Expression of Leaves in Full Opening Flower Stages of Hybrids & Their Parents and Heterosis in Pest-resistant Cotton. XING Chao-Zhu,ZHAO Yun-Lei,YU Shu-Xun,ZHANG Xian-Long,GUO Li-Ping,WANG Hai-Lin. 2006

[9]Strand-specific RNA-Seq transcriptome analysis of genotypes with and without low-phosphorus tolerance provides novel insights into phosphorus-use efficiency in maize. Du, Qingguo,Wang, Kai,Xu, Cheng,Zou, Cheng,Xie, Chuanxiao,Xu, Yunbi,Li, Wen-Xue. 2016

[10]Estimating Plasmodiophora brassicae gene expression in lines of B. rapa by RT-PCR. Wu, Li-yan,Li, Shi-kai,Gong, Ya-ju,Zhong, Li,He, Jiang-Ming,Siemens, Johannes,Ludwig-Mueller, Jutta. 2012

[11]Comparative Transcriptome Analysis Revealed Genes Commonly Responsive to Varied Nitrate Stress in Leaves of Tibetan Hulless Barley. Zeng, Xingquan,Wang, Yulin,Xu, Qijun,Yuan, Hongjun,Tang, Yawei,Nyima, Tashi,Wei, Zexiu,Zeng, Xingquan,Wang, Yulin,Xu, Qijun,Yuan, Hongjun,Tang, Yawei,Nyima, Tashi,Wei, Zexiu,Zeng, Xingquan,Wang, Yulin,Xu, Qijun,Yuan, Hongjun,Tang, Yawei,Qin, Cheng,Bai, Lijun. 2016

[12]Molecular characterization and expression analysis of the porcine caveolin-3 gene. Zhu, Zhengmao,Li, Yong,Mo, Delin,Li, Kui,Zhao, Shuhong.

[13]Molecular cloning and characterization of cotton cDNAs expressed in developing fiber cells. Guang-Rong ZHAO,Jin-Yuan LIU,Xiong-Ming DU. 2001

[14]Transcriptome analysis reveals the effect of pre-harvest CPPU treatment on the volatile compounds emitted by kiwifruit stored at room temperature. Luo, Jing,Guo, Linlin,Huang, Yunan,Wang, Chao,Qiao, Chengkui,Pang, Rongli,Li, Jun,Pang, Tao,Wang, Ruiping,Xie, Hanzhong,Fang, Jinbao. 2017

[15]Cytoplasmic polyhedrosis virus-induced differential gene expression in two silkworm strains of different susceptibility. Gao, Kun,Deng, Xiang-yuan,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie,Gao, Kun,Qian, He-ying,Qin, Guang-xing,Hou, Cheng-xiang,Guo, Xi-jie.

[16]Identification of differential gene expression profiles between winter dormant and sprouting axillary buds in tea plant (Camellia sinensis) by suppression subtractive hybridization. Wang, Xinchao,Hao, Xinyuan,Ma, Chunlei,Cao, Hongli,Yue, Chuan,Wang, Lu,Zeng, Jianming,Yang, Yajun,Wang, Xinchao,Hao, Xinyuan,Ma, Chunlei,Cao, Hongli,Yue, Chuan,Wang, Lu,Zeng, Jianming,Yang, Yajun,Hao, Xinyuan. 2014

[17]Osmotic responses of different strains of Steinernema carpocapsae. Yan, Xun,De Clercq, Patrick,Moens, Maurice,Yan, Xun,Han, Richou,Jones, John,Chen, Shulong,Moens, Maurice. 2011

[18]A gene family from Echinococcus granulosus differentially expressed in mature adult worms. Zhang, WB,Li, J,You, H,Zhang, ZZ,Turson, G,Loukas, A,McManus, DP. 2003

[19]Microarray analysis of gene expression in seeds of Brassica napus planted in Nanjing (altitude: 8.9 m), Xining (altitude: 2261.2 m) and Lhasa (altitude: 3658 m) with different oil content. Fu, San-Xiong,Qi, Cunkou,Cheng, Hao.

[20]Comparison of transcriptome under red and blue light culture of Saccharina japonica (Phaeophyceae). Wang, Wen-Jun,Wang, Fei-Jiu,Sun, Xiu-Tao,Liu, Fu-Li,Liang, Zhou-Rui.

作者其他论文 更多>>

微信小程序