Molecular characterization and expression analysis of the beta-actin gene from the ridgetail white prawn Exopalaemon carinicauda

文献类型： 外文期刊

第一作者： Liang, J. P.

作者： Liang, J. P.;Nie, G. X.;Liang, J. P.;Ge, Q. Q.;Li, J. T.;Liu, P.;Li, J.;Wang, Y.

作者机构：

关键词： Exopalaemon carinicauda;beta-actin;Ovarian development;Internal control;Gene expression

期刊名称：GENETICS AND MOLECULAR RESEARCH （ 影响因子：0.764； 五年影响因子：0.912 ）

ISSN： 1676-5680

年卷期： 2016 年 15 卷 2 期

页码：

收录情况： SCI

摘要： Actin is a highly conserved protein that is found in all eukaryotic cells, and has been widely used as an internal control gene in gene expression studies. In this study, we cloned an actin gene (named Ec beta-actin) from Exopalaemon carinicauda and determined its expression levels. The full-length cDNA of Ec beta-actin was 1335 bp long, comprising a 1131-bp ORF encoding 376 amino acids, a 65-bp 5'-UTR, and a 139-bp 3'-UTR with a poly(A) tail. The A + T content was approximately 79% in the 3'-UTR of the Ec beta-actin mRNA. The 3'-UTR contained two repeats of the AUUUA motif. The putative protein Ec beta-actin showed high identity (97-99%) with other actins

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