Linkage mapping of a dominant male sterility gene Ms-cd1 in Brassica oleracea

文献类型： 外文期刊

第一作者： Wang, XW

作者： Wang, XW;Lou, P;Bonnema, G;Yang, BJ;He, HJ;Zhang, YG;Fang, ZY

作者机构：

关键词： Brassica oleracea;dominant male-sterility gene;bulk segregant analysis;amplified fragment length polymorphism (AFLP);genetic mapping

期刊名称：GENOME （ 影响因子：2.166； 五年影响因子：2.474 ）

ISSN： 0831-2796

年卷期： 2005 年 48 卷 5 期

页码：

收录情况： SCI

摘要： The dominant male sterility gene Ms-cdl (c, cabbage; d, dominant) was identified as a spontaneous mutation in the spring cabbage line 79-399-3. The Ms-cd1 gene is successfully applied in hybrid seed production of several Brassica oleracea cultivars in China. Amplified fragment length polymorphism (AFLP), technology was used to identify markers linked to the Ms-cd1 gene in bulks of male-sterile and male-fertile individuals of a segregating BC3 population and in a near-isogenic population of 25 male-sterile plants. Twelve markers within a 20-cM interval proximal to the Ms-cd1 gene were identified, 5 of which can be used to select homozygous male-sterile Ms-cd1/Ms-cd1 plants. Three AFLP markers and 3 sequence characterized amplified region markers that were linked to MS-cd1 mapped onto linkage group O9, corresponding to chromosome 3 of B. oleracea. This region corresponds to the top of chromosome 5 in Arabidopsis thaliana.

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