Production of fertile intergeneric somatic hybrids between Brassica napus and Sinapis arvensis for the enrichment of the rapeseed gene pool

文献类型： 外文期刊

第一作者： Hu, Q

作者： Hu, Q;Andersen, SB;Dixelius, C;Hansen, LN

作者机构： Chinese Acad Agr Sci, Inst Oil Crops, Wuhan 430062, Peoples R China;Chinese Acad Agr Sci, Inst Oil Crops, Wuhan 430062, Peoples R China; Royal Vet & Agr Univ, Dept Agr Sci, DK-1871 Frederiksberg C, Denmark; Swedish Univ Agr Sci, Dept Plant Biol, S-75007 Uppsala, Sweden

关键词： blackleg resistance;intergeneric hybridization;protoplast fusion;Xinjiang wild mustard

期刊名称：PLANT CELL REPORTS （ 2020影响因子：4.57； 五年影响因子：4.463 ）

ISSN： 0721-7714

年卷期： 2002 年 21 卷 2 期

页码：

收录情况： SCI

摘要： Somatic hybrids between Brassica napus (oil-seed rape) and its wild relative Sinapis arvensis (Xinjiang wild mustard) from northwestern China were produced by fusing mesophyll protoplasts. Fifty-four plants were identified as symmetric hybrids and four as asymmetric hybrids by random amplified polymorphic DNA analysis and nuclear DNA content. The morphology of investigated 58 hybrid plants resembled characters from both parental species. Highly fertile hybrids were recovered where the fertility was associated with the choice of B. napus genotype. Enhanced disease resistance to Leptosphaeria maculans was found in S. arvensis and in the hybrid offspring. This plant material has great potentials not only for use as a bridge for the introduction of a number of valuable traits from the wild species to Brassica crops but also for breeding new varieties with improved blackleg resistance.

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