Plant regeneration from mesophyll protoplasts in Isatis indigotica

文献类型： 外文期刊

第一作者： Hu, Q

作者： Hu, Q;Andersen, SB;Hansen, LN

作者机构：

关键词： Cruciferae;medicinal crop;protoplast culture;rooting efficiency

期刊名称：PLANT CELL TISSUE AND ORGAN CULTURE （ 影响因子：2.711； 五年影响因子：2.73 ）

ISSN： 0167-6857

年卷期： 1998 年 55 卷 2 期

页码：

收录情况： SCI

摘要： Protoplasts of an accession of Isatis indigotica Fort. were isolated from mesophyll tissue by enzymatic digestion and cultured using a feeder cell system. Shoot regeneration efficiency was 100% via organogenesis among 627 isolated calluses within 30-37 days. Among these shoot initiating calluses, 162 (22.6%) developed normal shoots with multiple (2-5) shoots per callus. The remaining calluses developed only vitreous shoots. High concentration (5 mu M) of indole-3-butyric acid had a positive effect on rooting compared to low concentration (0.5 mu M) of indole-3-butyric acid and alpha-naphthalene-acetic acid. The average rooting efficiency of regenerated shoots of two experiments was higher on LS medium with 5 mu M indole-3-butyric acid than on LS medium without growth regulators. Twenty-nine plantlets, with 2-3 expanded leaves and roots were potted in soil and 22 developed normally to maturity in the glasshouse.

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