OsCNGC13 promotes seed-setting rate by facilitating pollen tube growth in stylar tissues

文献类型： 外文期刊

第一作者： Wang, Yihua

作者： Wang, Yihua;Wang, Jiachang;Yu, Yang;Wang, Yunlong;Zhang, Huan;Jiang, Ling;Wan, Jianmin;Xu, Yang;Ren, Yulong;Chen, Jun;Wang, Ying;Zhang, Xin;Guo, Xiuping;Wu, Fuqing;Zhu, Shanshan;Lin, Qibing;Wu, Chuanyin;Wang, Haiyang;Wan, Jianmin;Yang, Jie;Long, Yu

作者机构：

期刊名称：PLOS GENETICS （ 影响因子：5.917； 五年影响因子：6.284 ）

ISSN： 1553-7404

年卷期： 2017 年 13 卷 7 期

页码：

收录情况： SCI

摘要： Seed-setting rate is a critical determinant of grain yield in rice (Oryza sativa L.). Rapid and healthy pollen tube growth in the style is required for high seed-setting rate. The molecular mechanisms governing this process remain largely unknown. In this study, we isolate a dominant low seed-setting rate rice mutant, sss1-D. Cellular examination results show that pollen tube growth is blocked in about half of the mutant styles. Molecular cloning and functional assays reveals that SSS1-D encodes OsCNGC13, a member of the cyclic nucleotidegated channel family. OsCNGC13 is preferentially expressed in the pistils and its expression is dramatically reduced in the heterozygous plant, suggesting a haploinsufficiency nature for the dominant mutant phenotype. We show that OsCNGC13 is permeable to Ca2+. Consistent with this, accumulation of cytoplasmic calcium concentration ([Ca2+](cyt)) is defective in the sss1-D mutant style after pollination. Further, the sss1-D mutant has altered extracellular matrix (ECM) components and delayed cell death in the style transmission tract (STT). Based on these results, we propose that OsCNGC13 acts as a novel maternal sporophytic factor required for stylar [Ca2+](cyt) accumulation, ECM components modification and STT cell death, thus facilitating the penetration of pollen tube in the style for successful double fertilization and seed-setting in rice.

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