TsPKA-r: a potential immunodiagnostic antigen for the detection of porcine cysticercosis

文献类型： 外文期刊

第一作者： Liu, Guangxue

作者： Liu, Guangxue;Liang, Panhong;Zhang, Shaohua;Guo, Aijiang;Wang, Lijie;Zheng, Yadong;Luo, Xuenong

作者机构：

关键词： TsPKA-r;T. solium;iELISA;Immunodiagnostic antigens

期刊名称：ACTA TROPICA （ 影响因子：3.112； 五年影响因子：3.002 ）

ISSN： 0001-706X

年卷期： 2017 年 171 卷

页码：

收录情况： SCI

摘要： Cysticercosis, caused by metacestodes of Taenia solium, has a significant soci-economic impact and is of considerable importance in public health. However, there are no specific diagnostic antigens to distinguish between T. solim and Taenia hydatigena. In the present study, cAMP-dependent protein kinase regulatory subunit (TsPKA-r), an excretory/secretary (ES) antigen of T. solium, was used to establish a specific and sensitive diagnostic tool for detection of porcine cysticercosis. The full-length sequence encoding TsPKA-r was amplified by PCR, sequenced and then identified by bioinformatics. The fusion protein with 6 x His-tags was expressed in E. colt, purified by Ni Sepharose (TM) 6 Fast Flow and used to test reactionogenicity by immunoblotting. TsPKA-r based indirect enzyme-linked immunosorbent assays (iELISA) showed good performance in recognition of sera of pigs experimentally infected with T. solium metacestodes, with 93.88% sensitivity and 96.40% specificity. There were no cross-reactions against the sera from pigs experimentally infected with T. hydatigena, Toxoplasma gondii or Trichinella spiralis. These results indicate that the TsPKA-r is a promising immunodiagnostic antigen for detection of porcine cysticercosis.

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