Pilot study of large-scale production of mutant pigs by ENU mutagenesis

文献类型： 外文期刊

第一作者： Hai, Tang

作者： Hai, Tang;Cao, Chunwei;Zhang, Ying;Zheng, Qiantao;Zhang, Tao;Wang, Xianlong;Wang, Dayu;Qi, Meng;Hong, Qianlong;Zhang, Rui;Wang, Xiupeng;Jia, Qitao;Wang, Xiao;Qin, Guosong;Li, Yongshun;Luo, Ailing;Jin, Weiwu;Yao, Jing;Huang, Jiaojiao;Zhang, Hongyong;Li, Menghua;Xie, Xiangmo;Zheng, Xuejuan;Wang, Hongmei;Zhao, Jianguo;Zhou, Qi;Hai, Tang;Cao, Chunwei;Shang, Haitao;Mu, Yanshuang;Yang, Shulin;Zhang, Ying;Zheng, Qiantao;Zhang, Tao;Wang, Xianlong;Liu, Yu;Kong, Qingran;Li, Kui;Wang, Dayu;Qi, Meng;Hong, Qianlong;Zhang, Rui;Wang, Xiupeng;Jia, Qitao;Wang, Xiao;Qin, Guosong;Li, Yongshun;Luo, Ailing;Jin, Weiwu;Yao, Jing;Huang, Jiaojiao;Zhang, Hongyong;Li, Menghua;Xie, Xiangmo;Zheng, Xuejuan;Guo, Kenan;Wang, Qinghua;Zhang, Shibin;Li, Liang;Xie, Fei;Zhang, Yu;Weng, Xiaogang;Yin, Zhi;Hu, Kui;Cong, Yimei;Zheng, Peng;Zou, Hailong;Xin, Leilei;Xia, Jihan;Ruan, Jinxue;Li, Hegang;Zhao, Weiming;Yuan, Jing;Liu, Zizhan;Gu, Weiwang;Li, Ming;Wang, Yong;Shang, H

作者机构：

期刊名称：ELIFE （ 影响因子：8.14； 五年影响因子：9.056 ）

ISSN： 2050-084X

年卷期： 2017 年 6 卷

页码：

收录情况： SCI

摘要： N-ethyl-N-nitrosourea (ENU) mutagenesis is a powerful tool to generate mutants on a large scale efficiently, and to discover genes with novel functions at the whole-genome level in Caenorhabditis elegans, flies, zebrafish and mice, but it has never been tried in large model animals. We describe a successful systematic three-generation ENU mutagenesis screening in pigs with the establishment of the Chinese Swine Mutagenesis Consortium. A total of 6,770 G1 and 6,800 G3 pigs were screened, 36 dominant and 91 recessive novel pig families with various phenotypes were established. The causative mutations in 10 mutant families were further mapped. As examples, the mutation of SOX10 (R109W) in pig causes inner ear malfunctions and mimics human Mondini dysplasia, and upregulated expression of FBXO32 is associated with congenital splay legs. This study demonstrates the feasibility of artificial random mutagenesis in pigs and opens an avenue for generating a reservoir of mutants for agricultural production and biomedical research.

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