文献类型: 外文期刊
第一作者: Bao-Hong Zhang
作者: Bao-Hong Zhang;Fang Liu;Chang-Bing Yao
作者机构:
关键词: Gossypium hirsutum L.;plant regeneration;somatic embryogenesis
期刊名称:PLANT CELL TISSUE AND ORGAN CULTURE ( 影响因子:2.711; 五年影响因子:2.73 )
ISSN: 0167-6857
年卷期: 2000 年 60 卷 2 期
页码:
收录情况: SCI
摘要: An efficient in vitro plant regeneration system characterized by rapid and continuous production of somatic embryos using leaf and stem explants of abnormal seedling as an explant have been developed in Gossypium hirsutum L. Embryogenic callus and somatic embryos have been obtained directly from the explants of cotton abnormal seedlings. Plant growth regulators influenced the induction of cotton somatic embryogenesis. The optimal medium for direct somatic embryogenesis was modified MS medium supplemented with 0.1 mg l(-1) ZT and 2 g l(-1) activated carbon. On this medium, an average of 28.0 and 28.1 matured somatic embryos formed from per leaf and stem explants respectively. The highest frequency of somatic embryogenesis was 100%. The somatic embryos were converted into normal plantlets when cultured on modified MS medium supplemented with 0.1 mg l(-1) ZT. Upon transfer to soil, plants grew well and appeared normal. Plants could be regenerated within 60-80 days. The system of cotton somatic embryogenesis and plant regeneration described here will facilitate the application of plant tissue culture and genetic engineering on cotton genetic improvement.
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