Cloning of Schistosoma japonicum Chinese strain TPI gene and characterization of its expression product in Escherichia coli

文献类型： 外文期刊

第一作者： Cai, XZ

作者： Cai, XZ;Lin, JJ;Yang, GZ;Shi, FH;Shen, W;Cai, YM;Wu, XF

作者机构： Chinese Acad Agr Sci, Shanghai Inst Anim Parasitol, Shanghai 200232, Peoples R China;Chinese Acad Agr Sci, Shanghai Inst Anim Parasitol, Shanghai 200232, Peoples R China; Chinese Acad Sci, Shanghai Inst Biochem, Shanghai 200031, Peoples R China

关键词： triose-phosphate isomerase;Schistosoma japonicum;gene cloning;gene expression;immunological function

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 2020影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 0582-9879

年卷期： 1998 年 30 卷 5 期

页码：

收录情况： SCI

摘要： Triose-phosphate isomerase is an important candidate for schistosoma antigens. A 800 bp DNA fragment was amplified by RT-PCR from adult Schistosoma japonicum mRNA. Sequence analysis revealed that this fragment contained S. japonicum (Chinese strain) triose-phosphate isomerase gene. Then this gene was cloned into the expression vector pGEX-4T and subsequently expressed in Escherichia coli. The recombinant GST-fusion protein was purified by glutathione agarose affinity chromatography. Its molecular weight was determined to be 54 kD. The yield of expression was around 30 mg/L E. coli culture. Western blotting showed that the recombinant protein had good antigenicity which could be helpful for the making of anti-Schistosoma japonicum multi-valent recombinant vaccine.

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