A protocol for in vitro maturation and fertilization of sheep oocytes

文献类型: 外文期刊

第一作者: Wang, S

作者: Wang, S;Liu, Y;Holyoak, GR;Evans, RC;Bunch, TD

作者机构:

关键词: in vitro maturation;fertilization;sheep oocytes;follicle-stimulating hormone;human chorionic gonadotropin

期刊名称:SMALL RUMINANT RESEARCH ( 影响因子:1.611; 五年影响因子:1.813 )

ISSN: 0921-4488

年卷期: 1998 年 29 卷 1 期

页码:

收录情况: SCI

摘要: Two in vitro maturation (IVM) and fertilization (NF) protocols were evaluated with the objective of improving upon a protocol that will optimize NF-assisted reproduction in sheep. There was no attempt to compare possible confounding effects within protocol. The addition of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) to the maturation (IVM) medium significantly enhanced ovine oocyte maturation as compared to human chorionic gonadotropin (hCG) alone. Oocytes fertilized in vitro with spermatozoa treated with calcium ionophore A23187 and caffeine in Brackett and Oliphant medium (Protocol 1) significantly improved oocyte fertilization and embryo development in vitro than with spermatozoa separated by swim-up in synthetic oviduct fluid containing estrus ewe serum (Protocol 2). Embryos derived from oocytes matured in medium containing FSH and LH and fertilized with Protocol 1 were transferred to synchronized recipients and resulted in the birth of lambs. This protocol may be useful for assisted reproduction in sheep. (C) 1998 Elsevier Science B.V. All rights reserved.

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