文献类型: 外文期刊
第一作者: Wang, S
作者: Wang, S;Liu, Y;Holyoak, GR;Evans, RC;Bunch, TD
作者机构:
关键词: in vitro maturation;fertilization;sheep oocytes;follicle-stimulating hormone;human chorionic gonadotropin
期刊名称:SMALL RUMINANT RESEARCH ( 影响因子:1.611; 五年影响因子:1.813 )
ISSN: 0921-4488
年卷期: 1998 年 29 卷 1 期
页码:
收录情况: SCI
摘要: Two in vitro maturation (IVM) and fertilization (NF) protocols were evaluated with the objective of improving upon a protocol that will optimize NF-assisted reproduction in sheep. There was no attempt to compare possible confounding effects within protocol. The addition of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) to the maturation (IVM) medium significantly enhanced ovine oocyte maturation as compared to human chorionic gonadotropin (hCG) alone. Oocytes fertilized in vitro with spermatozoa treated with calcium ionophore A23187 and caffeine in Brackett and Oliphant medium (Protocol 1) significantly improved oocyte fertilization and embryo development in vitro than with spermatozoa separated by swim-up in synthetic oviduct fluid containing estrus ewe serum (Protocol 2). Embryos derived from oocytes matured in medium containing FSH and LH and fertilized with Protocol 1 were transferred to synchronized recipients and resulted in the birth of lambs. This protocol may be useful for assisted reproduction in sheep. (C) 1998 Elsevier Science B.V. All rights reserved.
分类号:
- 相关文献
作者其他论文 更多>>
-
Cloning, characterization, and expression analysis of hepcidin gene from red sea bream (Chrysophrys major)
作者:Chen, SL;Xu, MY;Ji, XS;Yu, GC;Liu, Y
关键词:
-
Aspects of soybean insect resistance breeding in China
作者:Wang, S
关键词:soybean;insect resistance;breeding
-
Preliminary study on kernel quality of high-yielding rice variety Te-San-Ai 2 grown in a simulated environment of elevated CO2 concentration
作者:Zhang, X;Liu, Y;Kong, Q;Huang, N;Chen, Y;Pan, D;Huang, LK;Wong, SC;Osmond, CB
关键词:
-
The effects of bovine serum albumin and fetal bovine serum on the development of pre- and postcleavage-stage bovine embryos cultured in modified CR2 and M199 media
作者:Wang, S;Liu, Y;Holyoak, GR;Bunch, TD
关键词:cattle-embryology;fetal bovine serum (FBS);bovine serum albumin (BSA);in vitro fertilization (IVF)
-
Fruit protected cultivation in China
作者:Gao, H;Wang, S;Wang, J
关键词:fruit;protected cultivation;greenhouse;environmental control
-
Bovine oocytes vitrified by the open pulled straw method and used for somatic cell cloning supported development to term
作者:Hou, YP;Dai, YP;Zhu, SE;Zhu, HB;Wu, TY;Gong, GC;Wang, HP;Wang, LL;Liu, Y;Li, R;Wan, R;Li, N
关键词:
-
Production of transgenic calves by somatic cell nuclear transfer
作者:Gong, GC;Dai, YP;Fan, BL;Zhu, HB;Wang, LL;Wang, HP;Tang, B;Liu, Y;Li, R;Wan, R;Huang, YH;Li, N
关键词:transgenic;somatic cell;nuclear transfer;EGFP;bovine