Production of transgenic blastocyst by nuclear transfer from different types of somatic cells in cattle

文献类型： 外文期刊

第一作者： Gong, GC

作者： Gong, GC;Dai, YP;Fan, BL;Zhu, HB;Wang, HP;Wang, LL;Fang, CG;Wan, R;Liu, Y;Li, R;Li, N

作者机构：

关键词： transgenic;nuclear transfer;somatic cells;EGFP;blastocyst;bovine

期刊名称：SCIENCE IN CHINA SERIES C-LIFE SCIENCES （ 影响因子：1.61； 五年影响因子：1.148 ）

ISSN：

年卷期：

页码：

收录情况： SCI

摘要： The present study examined the effects of genetic manipulation to the donor cell and different types of transgenic donor cells on developmental potential of bovine nuclear transfer (NT) embryos. Four types of bovine somatic cells, including granulosa cells, fetal fibroblasts, fetal oviduct epithelial cells and fetal ovary epithelial cells, were transfected with a plasmid (pCE-EGFP-lres-Neo-dNdB) containing the enhanced green fluorescent protein (EGFP) and neomycin-resistant (Neo~r) genes by electroporation. After 14 days selection with 800 μg/mL G418, transgenic cell lines from each type of somatic cells were obtained. Nontransgenic granulosa cells and all 4 types of transgenic somatic cells were used as nuclear donor to produce transgenic embryos by NT. There was no significant difference in development rates to the blastocyst stage for NT embryos from transgenic and nontransgenic granulosa cells (44.6% and 42.8%, respectively), and transfer of NT embryos derived from transgenic and nontransgenic granulosa cells to recipients resulted in similar pregnancy rates on day 90 (19% and 25%, respectively): The development rates to the blastocyst stage of NT embryos were significantly different among different types of transgenic donor cells (P<0.05). Blastocyst rates from fetal oviduct epithelial cell and granulosa cell (49.1% and 44.6%, respectively) were higher than those from fetal fibroblast (32.7%) and fetal ovary epithelial cell (22.5%). These results suggest that (ⅰ) genetic manipulation to donor cells has no negative effect on in vitro and early in vivo developmental competence of bovine NT embryos and (ⅱ) granulosa and fetal oviduct epithelial cells can be used to produce transgenic bovine NT embryos more efficiently. In addition, GFP can be used to select transgenic NT embryos as a non-invasive selective marker.

分类号： Q1

  • 相关文献

[1]Production of transgenic calves by somatic cell nuclear transfer. Gong, GC,Dai, YP,Fan, BL,Zhu, HB,Wang, LL,Wang, HP,Tang, B,Liu, Y,Li, R,Wan, R,Huang, YH,Li, N.

[2]Cell-cycle synchronization of fibroblasts derived from transgenic cloned cattle ear skin: effects of serum starvation, roscovitine and contact inhibition. Sun, XiuZhu,Lil, Ning,Wang, ShuHui,Wang, HaiPing,Wang, Lili,Ying, Liu,Li, Rong,Zhang, YunHai.

[3]Somatic cells serve as a potential propagule bank of Enteromorpha prolifera forming a green tide in the Yellow Sea, China. Zhang, Xiaowen,Mao, Yuze,Zhuang, Zhimeng,Wang, Qingyin,Ye, Naihao,Wang, Hongxia,Liang, Chengwei.

[4]In vitro development of donated frozen-thawed human embryos in a prototype static microfluidic device: a randomized controlled trial. Kieslinger, Dorit C.,Vergouw, Carlijn G.,Kostelijk, Elisabeth H.,Lambalk, Cornelis B.,Hao, Zhenxia,Le Gac, Severine,Hao, Zhenxia,Le Gac, Severine,Hao, Zhenxia.

[5]Optimization of cryopreservation of buffalo (Bubalus bubalis) blastocysts produced by in vitro fertilization and somatic cell nuclear transfer. Yang, C. Y.,Pang, C. Y.,Yang, B. Z.,Li, R. C.,Liang, X. W.,Lu, Y. Q..

[6]Progesterone and 17 beta-estradiol, but not follicle stimulating hormone, alter the sex ratio of murine embryos fertilized in vitro. Zhang, L.,Du, W.,Lin, X.,Zhang, L.,Zhang, A.,Chen, H.. 2008

[7]Expression of Foreign Genes Demonstrates the Effectiveness of Pollen-Mediated Transformation in Zea mays. Yang, Liyan,Cui, Guimei,Wang, Yixue,Hao, Yaoshan,Du, Jianzhong,Wang, Changbiao,Zhang, Huanhuan,Wu, Shu-Biao,Sun, Yi,Yang, Liyan,Zhang, Hongmei,Wu, Shu-Biao,Sun, Yi. 2017

[8]Construction of Rice Site-Specific Chloroplast Transformation Vector and Transient Expression of EGFP Gene in Dunaliella Salina. Han, Xiaoxia,Zuo, Jia,Xie, Lingling,He, Ronghua,Gao, Jing,Cao, Mengliang,Yuan, Longping,Cao, Mengliang,Chang, Lan,Cao, Mengliang.

[9]Generation of Recombinant Rabies Virus CVS-11 Expressing eGFP Applied to the Rapid Virus Neutralization Test. Xue, Xianghong,Zheng, Xuexing,Liang, Hongru,Feng, Na,Zhao, Yongkun,Gao, Yuwei,Wang, Hualei,Yang, Songtao,Xia, Xianzhu,Xue, Xianghong,Yang, Songtao,Xia, Xianzhu,Zheng, Xuexing,Xia, Xianzhu. 2014

[10]A Preliminary Study on Stable Transfection of EGFP in Buffalo Cumulus Cells. Shang, Jiang-Hua,Yang, Chun-Yan,Qin, Jing,Zheng, Hai-Ying,Huang, Fen-Xiang,Wang, Jian,Liu, Hua-Zhong,Liu, Hua-Zhong. 2013

[11]Inactivation of eGFP DNA in PK-15 cells via CRISPR/Cas9 system. Ishag, Hassan Z. A.,Liu, Maojun,Yang, Ruosong,Xiong, Qiyan,Feng, Zhixin,Shao, Guoqing,Liu, Maojun. 2017

[12]Development of a GFP-expressing Ustilaginoidea virens strain to study fungal invasion and colonization in rice spikelets. Andargie, Mebeaselassie,Li, Luoye,Li, Jianxiong,Feng, Aiqing,Zhu, Xiaoyuan.

[13]Cloning and sequence analysis of oilseed rape (Brassica napus) SHP2 gene. Tan, Xiao-Li,Qi, Cun-Kou,Tan, Xiao-Li,Xia, Zong-Wei,Zhang, Li-Li,Zhang, Zhi-Yan,Guo, Zhong-Jian.

[14]Effect of trichostatin A on transfected donor cells and subsequent development of porcine cloned embryos. Liu Di,Ma Hong,Wang Liang,Guo Zhen-hua,Li Zhong-qiu,Fu Bo,Liu Di,Fang Qing-chang,Ren Liang.

[15]Efficient production of omega-3 fatty acid desaturase (sFat-1)-transgenic pigs by somatic cell nuclear transfer. Pan DengKe,Zhang Li,Feng Chong,Long Chuan,Liu Xiao,Zhou YanRong,Chen HongXing,Wan Rong,Zhang Jian,Lin AiXing,Dong EnQiu,Wang ShuChen,Xu HouGang. 2010

[16]Nuclear transfer in leach (Paramisgurnus dabryanus Sauvage) by cell-to-cell electrofusion. Hongtuo, F,Chingjiang, W. 2001

[17]Birth of calves expressing the enhanced green fluorescent protein after transfer of fresh or vitrified/thawed blastocysts produced by somatic cell nuclear transfer. Gong, GC,Dai, YP,Fan, BL,Zhu, HB,Zhu, S,Wang, HP,Wang, LL,Tang, B,Li, R,Wan, R,Liu, Y,Huang, YH,Zhang, L,Sun, XZ,Li, N.

[18]Improvement of the nuclear transfer efficiency by using the same genetic background of recipient oocytes as the somatic donor cells in goats. Liu, Hai-jun,Zheng, Zi,Wang, Ru,Xue, Jun,Li, Kui,Ying, Zheng-Zhou.

[19]Sperm nuclear transfer and transgenic production in the fish medaka. Liu, Tongming,Hong, Yunhan,Liu, Ling,Wei, Qiwei,Hong, Yunhan.

[20]Effects of different states of sheep fetal fibroblasts as donor cells on the early development in vitro of reconstructed sheep embryos. Wang Hai,Ao Hong,Pan QiuZhen,Li RongQi,Zhao MengBin,Lian ZhengXing,Li Ning,Wu ChangXin.

作者其他论文 更多>>

微信小程序