Cloning of Schistosoma japonicum Chinese strain fatty acid binding protein (Sj-FABPc) gene and its overproduction in Escherichia coli

文献类型： 外文期刊

第一作者： Cai, XZ

作者： Cai, XZ;Tian, E;Yang, GZ;Shi, FH;Shen, W;Cai, YM;Wu, XF

作者机构：

关键词： fatty acid binding protein;Schistosoma japonicum;gene cloning;gene expression;vaccine

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 0582-9879

年卷期： 1998 年 30 卷 2 期

页码：

收录情况： SCI

摘要： A 600 bp DNA fragment was amplificated by PCR, from an adult Schistosoma japonicum cDNA library. Sequence analysis revealed that this fragment contained the S. japonicum Chinese Mainland strain fatty acid binding protein(Sj-14FABPc) gene. This gene was then cloned into the expression vector pGEX-2T,and subsequently expressed in Escherichia coli. The recombinant GST-fusion protein could be purified by glutathione agarose affinity chromatography. Its molecular weight was about 41 kD. The yield of expression was around 25 mg/L E. coli culture. The immunological test suggested that the recombinant protein had good antigenicity,and could be developed into a new vaccine molecule of S. japonicum.

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