Transgenic tobacco plants with a fully synthesized GFM CryIA gene provide effective tobacco bollworm (Heliothis armigera) control

文献类型： 外文期刊

第一作者： Huang, QM

作者： Huang, QM;Mao, LQ;Huang, WH;Guo, SD

作者机构：

关键词： GFM CryIA gene;transgenic tobacco plants;insect-resistance

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 1998 年 40 卷 3 期

页码：

收录情况： SCI

摘要： GFM CryIA gene is a fully modified synthetic gene derived from insecticidal crystal protein gene of Bacillus thuringiensis Berliner (Bt). It was synthesized based on the codon usage of plant genes instead of changing the primary sequences of amino acids of insecticidal crystal protein (ICP) gene of Bacillus thuringiensis Hubner. To test the function of the synthetic GFM CryIA gene, we introduced the GFM CryIA gene into tobacco plant cells via an Agrobacterium tumefaciens (Smith et Townsedn) Conn binary vector system. As expected, the GFM CryIA gene is expressed under control of the cauliflower mosaic virus (CaMV) 35S promoter and allows efficient production of lepidopteran insect-specific toxic proteins in the transformed tobacco plants. Bioassays using transgenic tobacco plants with tobacco bollworm showed that the transgenic tobacco plants expressing proteins of GFM CryIA gene had effective control to tobacco bollworm. In this paper the authors firstly report the complete synthesis of GFM CryIA gene and the construction of plant expression vector pGBI4AB. The authors performed introduction of the synthetic GFM CryIA gene into the tobacco plants, and the integration of GFM CryIA gene into tobacco genome was confirmed by Southern blot analysis of the tobacco genomic DNA. The gene was efficiently expressed in the transgenic tobacco plants and effective tobacco bollworm control was verified by the insect-bioassays.

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