Relationship between Omega as well as the length of 3 ' poly (dA) and efficiency of gene expression

文献类型： 外文期刊

第一作者： Mao, LQ

作者： Mao, LQ;Guo, SD

作者机构：

关键词： expression vector;Omega sequence;poly(dA);GUS gene;transient expression

期刊名称：ACTA BOTANICA SINICA （ 影响因子：0.599； ）

ISSN： 0577-7496

年卷期： 1998 年 40 卷 12 期

页码：

收录情况： SCI

摘要： Three plant high expression vectors harboring 25, 50 and 100 deoxyadenylate (dA) residues respectively in 3' untranslated region (3'-UTR) were constructed by inserting poly(dA) sequence into the primary vector containing CaMV 35S promoter doubled with region B and Omega which is a leader sequence derived from tobacco mosaic virus, within 5'-UTR. Transient expression of chimeric GUS gene in transgenic tobacco (Nicotiana tabacum L.) mesophyll protoplasts showed that:doubled enhancer, Omega and poly(dA) increased GUS expression. When both Omega and poly(dA) were present, the level of expression increased further, compared to that when only Omega was present. Moreover, when Omega Tvas present, doubling the length of poly(dA) resulted in a further increase in GUS expression, which suggested a positive relationship between poly(dA) length and the level of expression.

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