Nif gene transfer and expression in chloroplasts: Prospects and problems

文献类型: 外文期刊

第一作者: Dixon, R

作者: Dixon, R;Cheng, Q;Shen, GF;Day, A;DowsonDay, M

作者机构:

关键词: chloroplast;genetic engineering;nif genes;nitrogenase;plant transformation;plastid

期刊名称:PLANT AND SOIL ( 影响因子:4.192; 五年影响因子:4.712 )

ISSN: 0032-079X

年卷期: 1997 年 194 卷 1-2 期

页码:

收录情况: SCI

摘要: The engineering of plants capable of fixing their own nitrogen is an extremely complex task, requiring the coordinated and regulated expression of 16 nif genes in an appropriate cellular location. We suggest that plastids may provide a favourable environment for nif gene expression provided that the nitrogenase enzyme can be protected from oxygen damage. Using the non-heterocystous cyanobacteria as a model, we argue that photosynthesis could be temporally separated from nitrogen fixation in chloroplasts by restricting nitrogenase synthesis to the dark period. We report preliminary data on the introduction and expression of one of nitrogenase components, the Fe protein, in transgenic tobacco and Chlamydomonas reinhardtii. Finally we discuss potential avenues for further research in this area and the prospects for achieving the ultimate goal of expressing active nitrogenase in cereal crops such as rice.

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