Studies on the expression of the 28kD glutathione S-transferase gene from Schistosoma japonicum in the silkworm (Bombyx mori) larvae and insect cells

文献类型： 外文期刊

第一作者： Tian, E

作者： Tian, E;Yang, GZ;Cai, YM;Shi, FH;Wu, XF

作者机构：

关键词： glutathione S-transferase;Schistosoma japonicum;recombinant Bombyx mori nuclear polyhedrosis virus;gene expression

期刊名称：ACTA BIOCHIMICA ET BIOPHYSICA SINICA （ 影响因子：3.848； 五年影响因子：3.67 ）

ISSN： 0582-9879

年卷期： 1997 年 29 卷 1 期

页码：

收录情况： SCI

摘要： The gene of the 28kD glutathione S-transferase(GST) from the Chinese strain of Schistosoma japonicum had been expressed in the silkworm (Bombyx mori) cells and larvae by Bombyx mori nuclear polyhedrosis virus (BmNPV) vector which had been modified. The GST gene was inserted into the right position of the BmNPV genome as identified by Southern hybridization. The product was a 28kD protein, had GST activity and antigenicity. The yield in BmN cells(1 x 10(6)cells/ml) was 0.77mg and more than 5mg in a silkworm larvae. All the results showed that it is possible to develop the GST expressed in the silkworm larvae to a new kind of genetic engineered schistosomiasis vaccine.

分类号：

  • 相关文献

[1]Molecular cloning and expression of five glutathione S-transferase (GST) genes from Banana (Musa acuminata L. AAA group, cv. Cavendish). Wang, Zhuo,Huang, Suzhen,Jia, Caihong,Liu, Juhua,Zhang, Jianbin,Xu, Biyu,Jin, Zhiqiang. 2013

[2]Cloning and expression of gynecophoral canal protein gene of Schistosoma japonicum (Chinese strain). Jin, YM,Lin, JJ,Feng, XG,Zhang, L,Wu, XF,Zhou, YC,Cai, YM. 2002

[3]Cloning and expression of actin gene of Schistosoma japonicum Chinese strain. Zhu, JG,Lin, JJ,Feng, XG,Wu, XF,Zhou, YC,Cai, YM. 2000

[4]Analysis of early hepatic stage schistosomula gene expression by subtractive expressed sequence tags library. Wang, Xinzhi,Feng, XinGang,Fu, Zhiqiang,Jin, Yamei,Peng, Jinbiao,Lin, JiaoJiao,Gobert, Geoffrey N.. 2009

[5]Gene cloning, expression and vaccine testing of Schistosoma japonicum SjFABP. Liu, JM,Cai, XZ,Lin, JJ,Fu, ZQ,Yang, GZ,Shi, FH,Cai, YM,Shen, W,Taylor, MG,Wu, XF. 2004

[6]Cloning of Schistosoma japonicum Chinese strain TPI gene and characterization of its expression product in Escherichia coli. Cai, XZ,Lin, JJ,Yang, GZ,Shi, FH,Shen, W,Cai, YM,Wu, XF. 1998

[7]Cloning of Schistosoma japonicum Chinese strain fatty acid binding protein (Sj-FABPc) gene and its overproduction in Escherichia coli. Cai, XZ,Tian, E,Yang, GZ,Shi, FH,Shen, W,Cai, YM,Wu, XF. 1998

[8]A Phi-Class Glutathione S-Transferase Gene for Verticillium Wilt Resistance in Gossypium arboreum Identified in a Genome-Wide Association Study. Qian Gong;Zhaoen Yang,Li, Fuguang,Eryong Chen,Gaofei Sun,Shoupu He,Hamama Islam Butt,Chaojun Zhang,Xueyan Zhang,Zuoren Yang,Xiongming Du,Fuguang Li. 2018

[9]Glutathione S-transferases from the larval gut of the silkworm Bombyx mori: cDNA cloning, gene structure, expression and distribution. Gui, Zhong Zheng,Kim, Bo Yeon,Lee, Kwang Sik,Wei, Ya Dong,Sohn, Hung Dae,Jin, Byung Rae,Gui, Zhong Zheng,Wei, Ya Dong,Guo, Xijie. 2008

[10]Effect of Dietary Cadmium on the Activity of Glutathione S-Transferase and Carboxylesterase in Different Developmental Stages of the Oxya chinensis (Orthoptera: Acridoidea). Zhang, Y. P.,Song, D. N.,Wu, H. H.,Yang, H. M.,Zhang, J. Z.,Ma, E. B.,Guo, Y. P.,Zhang, Y. P.,Li, L. J.. 2014

[11]Effects of elevated CO2 on the nutrient compositions and enzymes activities of Nilaparvata lugens nymphs fed on rice plants. Wu Gang,Zhuang Jing,Zhao WanYun,Hua HongXia,Huang WenKun,Su Li,Li JunSheng,Xiao NengWen,Xiong YanFei. 2012

[12]Correlation between pesticide resistance and enzyme activity in the diamondback moth, Plutella xylostella. Gong, Ya-Jun,Wang, Ze-Hua,Shi, Bao-Cai,Kang, Zong-Jiang,Zhu, Liang,Jin, Gui-Hua,Wei, Shu-Jun. 2013

[13]Aphicidal Activity of Illicium verum Fruit Extracts and Their Effects on the Acetylcholinesterase and Glutathione S-transferases Activities in Myzus persicae (Hemiptera: Aphididae). Zhou, Ben-Guo,Wang, Sa,Dou, Ting-Ting,Liu, Su,Li, Mao-Ye,Li, Shi-Guang,Lin, Hua-Feng,Zhou, Ben-Guo,Hua, Ri-Mao. 2016

[14]Effects of excess copper on the oxidative stress in roots of maize seedlings. Wang, Yan Zhao,Nie, Li Hong,Tie, Shuanggui,Xie, Deyi,Zhu, Weihong,Qi, Jianshuang,Yue, Runqing. 2011

[15]Molecular cloning and oxidative stress response of a sigma-class glutathione S-transferase of the bumblebee Bombus ignitus. Kim, Bo Yeon,Hui, Wei Li,Lee, Kwang Sik,Wan, Hu,Jin, Byung Rae,Hui, Wei Li,Chen, Shan,Yoon, Hyung Joo,Gui, Zhong Zheng.

[16]The silkworm GSTe4 is sensitive to phoxim and protects HEK293 cells against UV-induced cell apoptosis. Liu, J.,Zhang, C. D.,Li, Y. F.,Liu, T. H.,Wang, L.,Lu, C.,Pan, M. H.,Liu, J.,Zhang, C. D.,Yu, Q. Y.,Zhang, Y. H.,Lu, C.,Pan, M. H..

[17]Glutathione S-transferases are involved in thiamethoxam resistance in the field whitefly Bemisia tabaci Q (Hemiptera: Aleyrodidae). Yang, Xin,He, Chao,Xie, Wen,Liu, Yating,Xia, Jixing,Yang, Zezong,Guo, Litao,Wen, Yanan,Wang, Shaoli,Wu, Qingjun,Yang, Fengshan,Zhou, Xiaomao,Zhang, Youjun.

[18]Induction effects of host plants on insecticide susceptibility and detoxification enzymes of Bemisia tabaci (Hemiptera: Aleyrodidae). Xie, Wen,Wang, Shaoli,Wu, Qingjun,Feng, Yuntao,Pan, Huipeng,Jiao, Xiaoguo,Zhou, Long,Yang, Xin,Fu, Wei,Teng, Haiyuan,Xu, Baoyun,Zhang, Youjun.

[19]De novo analysis of the Tenebrio molitor (Coleoptera: Tenebrionidae) transcriptome and identification of putative glutathione S-transferase genes. Liu, Su,Shi, Xiao-Xiao,Jiang, Yan-Dong,Zhu, Zi-Jie,Qian, Ping,Zhang, Min-Jing,Yu, Hang,Zhu, Qing-Zi,Zhu, Zeng-Rong,Gong, Zhong-Jun.

[20]Effects of Insect Viruses and Pesticides on Glutathione S-Transferase Activity and Gene Expression in Bombyx mori. Gui, Zhongzheng,Hou, Chengxiang,Liu, Ting,Qin, Guangxin,Li, Muwang,Gui, Zhongzheng,Hou, Chengxiang,Liu, Ting,Qin, Guangxin,Li, Muwang,Jin, Byungrae.

作者其他论文 更多>>

微信小程序