Efficient RNA interference for three neuronally-expressed genes in Nilaparvata lugens (Stal) (Hemiptera: Delphacidae)

文献类型： 外文期刊

第一作者： Xu, Chen

作者： Xu, Chen;Guo-Qing, Li;Xu, Chen;Pin-Jun, Wan;Qiang, Fu

作者机构：

关键词： Nilaparvata lugens;RNA interference;Efficiency;Neuronally-expressed gene

期刊名称：JOURNAL OF ASIA-PACIFIC ENTOMOLOGY （ 影响因子：1.303； 五年影响因子：1.427 ）

ISSN： 1226-8615

年卷期： 2017 年 20 卷 2 期

页码：

收录情况： SCI

摘要： In the brown planthopper Nilaparvata lugens, introduction of double-stranded RNA (dsRNA) successfully knocks down genes transcribed in several types of tissues. However, whether RNA interference (RNAi) can effectively reduce the transcripts of neuronally-expressed genes remains undetermined. In the present paper, we selected three neuronally-expressed genes respectively encoding prothoracicotropic hormone (NIPTTH), pigment-dispersing factor (NIPDF) and short neuropeptide F (NIsNPF). The transcripts of the three genes were detectable throughout all tested developmental stages, and were mainly restricted to the nervous system of N. lugens. We introduced dsRNA originated from each of the three neuronally-expressed genes at a dose of 300 ng into the nymphal body cavity by microinjection. The target mRNA was successfully knocked down. Moreover, silencing of NIPTTFI downregulated the transcripts of two ecdysteroidogenesis genes (NIPHM and NIDIB), and surpressed the expression of an ecdysone-response genes (NIFTZ-FI). Furthermore, RNAi of NIPTTH caused typical 20-hydroxyecdysone deficient phenotypes: nymphal development was delayed and nymphal lethality occurred. In addition, injection of a series of dsNIsDPF solutions at the doses of 300, GO, 12, 2.4 and 0.5 ng into the fourthinstar nymphs decreased its target transcript in a dose-dependent manner: the mRNA levels reduced by 94%, 86%, 57%, 33% and 29%, respectively. Therefore, delivery of dsRNA by microinjection can effectively repress the transcripts of the neuronally-expressed genes in N. lugens. (C) 2017 Published by Elsevier B.V. on behalf of Korean Society of Applied Entomology, Taiwan Entomological Society and Malaysian Plant Protection Society.

分类号：

  • 相关文献

[1]Glycogen Phosphorylase and Glycogen Synthase: Gene Cloning and Expression Analysis Reveal Their Role in Trehalose Metabolism in the Brown Planthopper, Nilaparvata lugens St(a)over-circlel (Hemiptera: Delphacidae). Zhang, Lu,Wang, Huijuan,Chen, Jianyi,Shen, Qida,Wang, Shigui,Tang, Bin,Xu, Hongxing. 2017

[2]Gene knockdown by intro-thoracic injection of double-stranded RNA in the brown planthopper, Nilaparvata lugens. Ding, Zhiping,Liu, Zewen,Liu, Shuhua,Yang, Baojun,Zhang, Chengwei.

[3]Knockdown of a putative argininosuccinate lyase gene reduces arginine content and impairs nymphal development in Nilaparvata lugens. Yuan, San-Yue,Li, Guo-Qing,Mu, Li-Li,Yuan, San-Yue,Wan, Pin-Jun,Fu, Qiang,Lai, Feng-Xiang.

[4]Efficiency, economics, and environmental implications of phosphorus resource use and the fertilizer industry in China. Oenema, Oene,Zhang, Weifeng,Fan, Mingsheng,Zhang, Fusuo,Ma, Wenqi,Ji, Yuexiu. 2008

[5]Regeneration and transformation of Crambe abyssinica. Qi, Weicong,Tinnenbroek-Capel, Iris E. M.,Schaart, Jan G.,Cheng, Jihua,Visser, Richard G. F.,Van Loo, Eibertus N.,Krens, Frans A.,Qi, Weicong,Huang, Bangquan,Cheng, Jihua. 2014

[6]Impact of sulfate nutrition on the utilization of atmospheric SO2 as sulfur source for Chinese cabbage. Yang, Liping,Stulen, Ineke,De Kok, Luit J..

[7]Changes in the efficiency of fertiliser use in China. Jin, Jiyun,Jin, Jiyun. 2012

[8]Increasing the efficiency of CRISPR-Cas9-VQR precise genome editing in rice. Hu, Xixun,Liu, Qing,Wang, Kejian,Meng, Xiangbing,Li, Jiayang,Meng, Xiangbing,Li, Jiayang,Li, Jiayang. 2018

[9]Energy requirements of Dorper crossbred ewe lambs. Deng, K. D.,Jiang, C. G.,Tu, Y.,Zhang, N. F.,Liu, J.,Ma, T.,Zhao, Y. G.,Xu, G. S.,Diao, Q. Y.,Deng, K. D..

[10]The selection of ELISA cut-off points for testing antibody to Newcastle disease by two-graph receiver operating characteristic (TG-ROC) analysis. Xu, H,Lohr, J,Greiner, M. 1997

[11]Energy Requirements for Maintenance and Growth of German Mutton Merino Crossbred Lambs. Deng Kai-dong,Diao Qi-yu,Jiang Cheng-gang,Tu Yan,Zhang Nai-feng,Liu Jie,Ma Tao,Zhao Yi-guang,Xu Gui-shan,Deng Kai-dong. 2013

[12]Metabolizable protein requirements of Dorper crossbred ram lambs. Deng, K. D.,Ma, T.,Jiang, C. G.,Tu, Y.,Zhang, N. F.,Liu, J.,Zhao, Y. G.,Xu, G. S.,Diao, Q. Y.,Deng, K. D..

[13]Nitrogen balance and groundwater nitrate contamination: Comparison among three intensive cropping systems on the North China Plain. Ju, XT,Kou, CL,Zhang, FS,Christie, P.

[14]Manganese availability and microbial populations in the rhizosphere of wheat genotypes differing in tolerance to Mn deficiency. Marschner, P,Fu, QL,Rengel, Z.

[15]Coupled Effects of Soil Water and Nutrients on Growth and Yields of Maize Plants in a Semi-Arid Region. Sun Zhan-Xiang,Zheng Jia-Ming,Sun Wen-Tao.

[16]Effects of elevated CO2 on the nutrient compositions and enzymes activities of Nilaparvata lugens nymphs fed on rice plants. Wu Gang,Zhuang Jing,Zhao WanYun,Hua HongXia,Huang WenKun,Su Li,Li JunSheng,Xiao NengWen,Xiong YanFei. 2012

[17]Research priorities for rice pest management in tropical asia: A simulation analysis of yield losses and management efficiencies. Willocquet, L,Elazegui, FA,Castilla, N,Fernandez, L,Fischer, KS,Peng, SB,Teng, PS,Srivastava, RK,Singh, HM,Zhu, DF,Savary, S. 2004

[18]Comparison of the effects of brown planthopper, Nilaparvata lugens (Stal) (Homoptera : Delphacidae), and rice leaffolder, Cnaphalocrocis medinalis Guenee (Lepidoptera : Pyralidae), infestations and simulated damage on nutrient uptake by the roots of rice plants. Yin, JL,Wu, JC,Yu, YS,Liu, JL,Xie, M,Wan, FH. 2005

[19]A Genome-Wide Identification and Analysis of the Basic Helix-Loop-Helix Transcription Factors in Brown Planthopper, Nilaparvata lugens. Wan, Pin-Jun,Yuan, San-Yue,Wang, Wei-Xia,Chen, Xu,Lai, Feng-Xiang,Fu, Qiang. 2016

[20]Influence of rice black streaked dwarf virus on the ecological fitness of non-vector planthopper Nilaparvata lugens (Hemiptera: Delphacidae). Xu, Hong-Xing,Zheng, Xu-Song,Yang, Ya-Jun,Lu, Zhong-Xian,He, Xiao-Chan. 2014

作者其他论文 更多>>

微信小程序